CXCL9是一个缺乏ELR结构域的趋化因子α亚家族的成员，最初被确定为小鼠巨噬细胞中的淋巴因子激活基因。随后以小鼠MIG cDNA为探针克隆了人CXCL9。CXCL9基因在中枢神经系统的巨噬细胞和初级胶质细胞中被诱导，特别是在ifn - γ的反应中。CXCL9已被证明是活化T淋巴细胞和TIL的趋化剂，但对中性粒细胞或单核细胞则不是。人类CXCL9 cDNA编码一个125个氨基酸残基前体蛋白和一个22个氨基酸残基信号肽，该信号肽切割后生成一个103个氨基酸残基成熟蛋白。CXCL9具有一个扩展的羧基端，包含超过50%的碱性氨基酸残基，比大多数其他趋化因子都要大。CXCL9的羧基末端残基容易发生蛋白水解裂解，导致天然和重组CXCL9的大小不均匀。在钙通量测定中，羧基端大量缺失的CXCL9被证明具有较低的活性。一种特异性于CXCL9和IP-10的趋化因子受体(CXCR3)已被克隆，并显示在il -2激活的T淋巴细胞中高度表达。在研发系统中生产的大肠杆菌表达CXCL9制剂已被证明含有超过80%的全长CXCL9。 Purity >97% SDS-PAGE. Purity is greater than 97% as determined by reducing SDS-PAGE Function Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. CXCL9, a member of the alpha subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T‑lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy‑terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy‑terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy‑terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes. The E. coli‑expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.
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Recombinant Human CXCL9 Protein
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| Chinese alias | 重组人CXCL9蛋白 | 重组人 CXCL9 蛋白 | ||
| English alias | Chemokine (C-X-C motif) ligand 9 | CMK | crg-10 | C-X-C motif chemokine 9 | CXCL9 | Gamma-interferon-induced monokine | Humig | MIG | MIGSmall-inducible cytokine B9 | monokine induced by gamma interferon | SCYB9Monokine induced by interferon-gamma | Recom | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无动物源 |
| PSA | - | logp | - |
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