compound introduction
Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.
