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Cytombritetm cell membrane red fluorescent probe

CAS number:unknown    molecular formula:

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compound introduction

CytoMBriteTM 细胞膜红色荧光探针(DiD)是一种亲脂性碳菁类染料,能够有效、稳定地标记质膜及细胞内膜结构。它具有细胞毒性低且不会在细胞间转移的特性,常被作为细胞融合、粘附、迁移的示踪分子,与PKH类染料相比,CytoMBrite染料使用方便、着色均匀。DiI(橙色荧光)、DiO(绿色荧光)、DiD(红色荧光)和其它细胞膜荧光染料如DiR(近红外荧光)、NIR680(远红外染料)配合使用,为多色成像和流式细胞分析提供了有效的工具。 CytoMBrite系列染料也可以用于甲醛固定后细胞的染色,同时兼容在染色后的甲醛固定步骤。此系列染料不适用于细菌或酵母。 以每次使用100 μL染色工作液计算,200 μL原液可以用400次。 产品参数: Ex/Em (MeOH) = 644/663 nm 分子式:C67H103ClN2O3S 分子量:1052.1 注意事项: 1. 使用前请将产品瞬时离心至管底,再进行后续实验。 2. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。 3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 应用范围: 细胞膜染料 Product introduction: Cytombritetm cell membrane red fluorescent probe (did) is a lipophilic carbocyanine dye, which can effectively and stably label the plasma membrane and intracellular membrane structures. It has the characteristics of low cytotoxicity and will not transfer between cells. It is often used as a tracer molecule for cell fusion, adhesion and migration. Compared with PKH dyes, cytombrite dyes are convenient to use and evenly colored. DiI (orange fluorescence), DIO (green fluorescence), did (red fluorescence) and other cell membrane fluorescent dyes, such as dir (near-infrared fluorescence) and nir680 (far-infrared dye), provide effective tools for multicolor imaging and flow cytometry analysis. Cytombrite series dyes can also be used for the staining of cells after formaldehyde fixation, and are compatible with the formaldehyde fixation step after staining. This series of dyes is not suitable for bacteria or yeast. At 100 per use μ L dyeing working solution calculation, 200 μ L stock solution can be used for 400 times. Product parameters: Ex/em (MeOH) = 644/663 nm molecular formula: c67h103cln2o3s molecular weight: 1052.1 Matters needing attention: 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching. 3. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application: Cell membrane dye

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