compound introduction
通用 Western 印迹法方案：糖蛋白样本量：500 ng凝集素浓度：0.1 μg/mL每个泳道加入500 ng糖蛋白样品运行 4-20％Bis-Tris SDS 页面凝胶将凝胶转移至 PVDF 膜上用 RTA 缓冲液（R0278 Sigma）在室温下封闭膜 1 小时在室温下用 RIPA 缓冲液将 HRP 凝集素以 0.1ug / ml 孵育 2 小时用 25ml RIPA 缓冲液洗涤膜 5×5 分钟使用化学发光底物（CPS1-120）进行检测 General Western Blot Protocol: Glycoprotein sample size: 500ng Lectin Concentration: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane Run 4-20% Bis-Tris SDS page gel Transfer gel to a PVDF membrane Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT Wash membrane 5 x 5 minutes with 25ml RIPA buffer Detect using chemiluminescent substrate (CPS1-120)
