来源于脑膜脓毒性黄杆菌（Elizabethkingia meningoseptica） 的PNGase F已被用于:寨卡20病毒E蛋白的去N-糖基化采用表达 CAR 的 COS 细胞评估柯萨奇病毒和腺病毒受体的糖基化验证 MHC 1 类多肽相关序列 A（MICA）的 N连接糖基化适用于蛋白质组学和糖生物学；与MALDI-TOF MS分析相容用于使蛋白质去糖基化。应用： 蛋白质组学级糖苷酶F经过从稀磷酸钾缓冲液中充分纯化和冻干，以生产出稳定的产品。 本品不含甘油和其他稳定剂，并且缓冲盐含量非常低。 这种高度纯化的材料非常适用于凝胶、溶液或印迹膜中糖蛋白或糖肽的N-连接去糖基化。 Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes. Application: PNGase F from Elizabethkingia meningoseptica has been used: for de- N -glycosylation of Zika20virus E protein to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells to verify the N -linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA) Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis Used to deglycosylate protein.
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糖苷酶F 来源于脑膜脓毒性伊丽莎白菌
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| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | BioReagent |
| PSA | - | logp | - |
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