多粘菌素B酶

多粘菌素B酶是采用重组基因的水溶性蛋白，呈深褐色液体。 根据多粘菌素B的化学结构而构建的重组基因蛋白。其基因片断的 活性中心，可破坏多粘菌素B的化学结构式，使开环/断链后失去该药物的抗菌性。 做抗生素的无菌检查时，应用手工注射器吸取无菌水注入药物的西林瓶中，摇匀，溶解，再吸出。加入至0.9%氯化钠的500ml溶液中，摇匀，不可用培养器上的针头溶解，输入，避免高浓度的溶液 通过滤膜，造成难以冲洗彻底。 这里，再次强调：做抗生素的无菌检查时，一定要手工注射器 注入无菌水溶解样品，再把溶解的样品转移至0.9%氯化钠的500ml溶液中摇匀，使得样品不会造成局部浓度过高，通过滤膜时难以冲洗彻底的情况。 无菌检验时，取酶3ml加入无菌水2ml，摇匀，使成多粘菌素B酶的稀释液，取2ml酶的稀释液放入1500ml的冲洗液摇匀。待冲洗液冲洗培养器的滤膜完成后，泵尽。用手工注射器在三个培养器的呼吸口刺入，各加1ml的稀释酶，尽量让酶平铺在滤膜整个面， 静止10分钟，使高浓度的酶与培养器滤膜充分接触，以破坏（中和、灭活）滤膜上残留的多粘菌素B药物，然后再泵入相应的培养基， 摇匀。阳性对照管加入1ml的大肠杆菌（100CFU/ml）。 在冲洗液加2ml的多粘菌素B酶的稀释液，可以去除滤膜内部 残留的多粘菌素B的少量药物抗菌性。 在三个培养器加1ml的多粘菌素B酶的稀释液，可以去除培养器内部壁上和滤膜表面残留多粘菌素B的少量药物抗菌性。 每天上午、下午各对阳性对照管轻轻摇匀一次。 客户可根据上述做方法学验证，也可根据实际操作情况做验证。 Polymyxin B enzyme is a water-soluble protein using recombinant genes and is a dark brown liquid. Recombinant gene protein based on the chemical structure of polymyxin B. The active center of its gene fragment can destroy the chemical structural formula of polymyxin B, so that the antimicrobial properties of the drug are lost after ring-opening/chain breaking. When doing sterility check of antibiotics, use a manual syringe to absorb sterile water and inject it into the drug vial, shake well, dissolve, and then suck it out. Add to 500ml solution of 0.9% sodium chloride, shake well, do not dissolve with the needle on the incubator, input, avoid high concentration of solution through the filter membrane, resulting in difficult to rinse thoroughly. Here, it is emphasized again: when doing sterile examination of antibiotics, it is necessary to inject sterile water into the manual syringe to dissolve the sample, and then transfer the dissolved sample to 500ml solution of 0.9% sodium chloride, so that the sample will not cause local concentration too high, difficult to wash thoroughly through the filter membrane. During sterility test, take 3ml of enzyme and add 2ml of sterile water and shake well to make diluent of polymyxin B enzyme. Take 2ml of enzyme diluent and add 1500ml of rinsing solution and shake well. After the rinse solution has washed the filter membrane of the incubator, the pump is exhausted. Use a manual syringe to Pierce the respiratory mouth of the three incubators, add 1ml of diluent enzyme to each of them, spread the enzyme on the entire surface of the filter membrane as much as possible, rest for 10 minutes, and make the high concentration of enzyme fully contact with the filter membrane of the incubator, so as to destroy (neutralize and inactivate) the residual polymyxin B drug on the filter membrane, and then pump into the corresponding medium and shake well. Positive pairs were treated with 1ml of E. coli (100CFU/ml). Adding 2ml dilution of polymyxin B enzyme to the rinse solution can remove a small amount of antimicrobial activity of polymyxin B remaining in the filter membrane. Adding 1ml dilution of polymyxin B enzyme to three incubators can remove a small amount of antimicrobial activity of polymyxin B remaining on the inner wall of the incubators and on the surface of the filter membrane. Shake the positive pair gently once a day in the morning and afternoon. Customers can do methodological verification according to the above, but also according to the actual operation of the verification.

No numbering system information yet

No physical and chemical property information yet

No safety information yet

No production method and use information yet

No upstream information yet

No downstream information yet

No related product information yet