核糖核酸酶 H (RNase H)

核糖核酸酶 H (RNase H) 已用于：体内 RNA 引发的 DNA 合成初始化第二链 cDNA 合成过程中 mRNA 消除RNA 的位点特异性切割检测天然来源的双链DNA中的RNA:DNA区域如果存在 oligo (dT)，则去除 mRNA 的 poly (A) 序列RNA提取和定量逆转录聚合酶链式反应(RT-PCR)应用： 核糖核酸酶H（RNA酶H）是一种位于细胞核与细胞质中的非特异性核糖核酸内切酶，。它普遍存在并广泛分布于病毒和人类等多种生物中。非（序列）专一性核糖核酸内切酶，可特异性地水解RNA:DNA杂合链中的RNA链。需要至少4个连续碱基对(RNA:DNA)才能发挥酶活性。RNase H可切割RNA释放出5′-寡聚核糖核苷酸。来源：大肠杆菌H560 pol A1存储液：25 mM Tris-HCl，50 mM KCl，1 mM 二硫苏糖醇，0.1 mM EDTA，50% 甘油 (v/v)，pH 8.0 (+ 4°C)单位体积活力：1 x 103 U/ml，按照Hillenbrand & Staudenbauer法分析。 Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. Source: E. coli H560 pol A1 Storage Buffer : 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C) Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer. Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human. Application: Ribonuclease H (RNase H) has been used for: In vivo RNA-primed initiation of DNA synthesis Elimination of mRNA during second-strand cDNA synthesis Site-specific cleavage of RNA Detection of RNA:DNA regions in double-stranded DNA of natural origin Removal of poly (A) sequences of mRNA if oligo (dT) is present RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)

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