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Bm-紫

CAS number:unknown    molecular formula:

overview

compound introduction

比色AP底物,可用于:免疫组化Western印迹Southern印迹Northern印迹菌落和噬菌斑杂交原位 杂交应用: BM紫是碱性磷酸酶(AP)的显色底物,其设计用于沉淀酶免疫测定。它可在膜或固体支持物上的AP结合位点处形成永久的深紫色条带或斑点。该试剂不需要进行混合或溶解,其是一种稳定的底物溶液可直接从瓶中使用。与使用BCIP/NBT双瓶系统相比,它产生更少的背景染色,且不会降低灵敏度。反应产物呈深紫色,不溶于水。形成的深紫色可通过视觉观察到。使用前外观: 淡黄色; 颜色显影后:深紫色 BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen. Appearance prior to use: light yellow color; After color development: dark purple Application: Colorimetric AP substrate for: Immunohistocytochemistry Western blot Southern blot Northern blot Colony and plaque hybridization In situ hybridization

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CAS numberunknownmolecular formula
molecular weight-Exact massRoche, pkg of 100 mL
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