细胞活化因子(BAFF,或BLyS)是调节B淋巴细胞存活和成熟细胞因子,属于TNF家族成员,能调节B细胞存活、增殖、发育和分化。作为近年来发现对B细胞生存分化具有重要作用因子,BAFF通过结合于三种不同受体发挥促B细胞分化成熟、类别转换、促进体液免疫应答及参与T细胞活化等功能。 一、检测原理: 阿拉丁生产的ELISA试剂盒采用“夹心法”:将捕获抗体包被于酶标板上,捕获样品及标准品中的靶蛋白,生物素化的检测抗体与靶蛋白结合,SABC复合物与生物素化检测抗体结合,形成免疫复合物,加入TMB显色液后,若反应孔中有靶蛋白则显蓝色,加入终止液变黄色,检测过程中未结合的成分均被洗去,用酶标仪在450nm处测OD值,靶蛋白浓度与OD值之间呈正比,通过绘制标准曲线计算出标本中靶蛋白的浓度,从而对检测样品进行定性或相对定量分析。 二、试剂盒组分: H1127430
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Human BAFF/BLyS/TNFSF13B ELISA Kit
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 人B细胞刺激因子酶联免疫吸附检测试剂盒 | ||
| English alias | Human BAFF/BLyS/TNFSF13B Enzyme-Linked ImmunoSorbent Assay Kit | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 生物活性 |
| PSA | - | logp | - |
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