ASR-490 reduces the viability of HCT116 and SW620 cells by downregulating Notch1 signaling. ASR-490 overcomes Notch1 overexpression and inhibits the growth of HCT/Notch1 transfectants. ASR-490 inhibits the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice In Vitro ASR-490 (0-1.6 µM; 24 h, 48 h) reduces the viability of HCT116 and SW620 cells in 24h (IC 50 =750 nM in HCT116 cells, IC 50 =1.2 µM in SW620 cells) and 48h (IC 50 =600 nM in HCT116 cells, IC 50 =850 nM in SW620 cells). ASR-490 (750 nM in HCT116 cells and 1.2 µM in SW620 cells, 24 h) shows apoptotic cell death and upregulation of the proapoptotic markers Bax and cleaved PARP Expression; inhibits the capability of colorectal cancer cells. ASR-490 (HCT116 cells; 750 nM in 24 h, 600 nM in 48 h) overcomes Notch1 overexpression and inhibits the growth of HCT/Notch1 transfectants. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HCT116, SW620 cells Concentration: 0-1.6 µM Incubation Time: 24 h, 48 h Result: Reduced the viability of HCT116 and SW620 cells in 24h (IC 50 =750 nM in HCT116 cells, IC 50 =1.2 µM in SW620 cells) and 48h (IC 50 =600 nM in HCT116 cells, IC 50 =850 nM in SW620 cells). Apoptosis AnalysisCell Line: HCT116, SW620 cells Concentration: 750 nM in HCT116 cells and 1.2 µM in SW620 cells Incubation Time: 24 h Result: Showed apoptotic cell death and upregulation the proapoptotic markers Bax and cleaved PARP Expression, inhibited the capability of colorectal cancer cells. Cell Proliferation AssayCell Line: HCT116 cells Concentration: 750 nM in 24 h, 600 nM in 48 h Incubation Time: 24 h, 48 h Result: Overcome Notch1 overexpression and inhibited the growth of HCT/Notch1 transfectants. In Vivo ASR-490 (5 mg/kg; i.p.; thrice a week for 4 weeks) inhibits the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Six- to 8-week-old BALB/c athymic nude mice (nu/nu) (pCMV/HCT116 and Notch1/HCT116 (C4) xenografts) . Dosage: 5 mg/kg Administration: i.p., thrice a week for 4 weeks Result: Inhibited the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice. Form:Solid IC50& Target:Notch1
All
Chemical Reagents
Biomedicine
Organic raw materials
Inorganic chemical industry
intermediate
Agricultural chemicals
Auxiliaries and catalysts
Fragrances and Flavors
Dyes and Pigments
Daily chemical industry
ASR-490
CAS number：2690312-67-3 molecular formula：C34H41NO7
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | - | ||
| CAS number | 2690312-67-3 | molecular formula | C34H41NO7 |
| molecular weight | 575.69 g/mol | Exact mass | ≥99% |
| PSA | - | logp | - |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
No safety information yet
Production methods and uses
No production method and use information yet
Related
upstream information
No upstream information yet
downstream information
No downstream information yet
MSDS
Found 0 shareMSDS
