ABTS One Component HRP Microwell Substrate

The ABTS (2,2’-azino-bis [3-ethylbenzthiazoline-6-sulfonic acid]) substrate is supplied as a one component, ready-to-use solution for ELISA applications. A soluble blue-green reaction product is obtained when this substrate system is reacted with horseradish peroxidase. Usage suggestions The product should be allowed to reach room temperature (25°C) prior to use. 1) For immunoassay microwell applications, 100 µl of substrate solution is added to each well producing a soluble, blue‐green product.  Color development time can range between 10‐40 minutes depending on the assay.  It is recommended that development time be optimized for your assay conditions. 2) For best results, sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units.  Sample OD values can be read with or without the addition of stop solution in the 405‐410 nm range. The substrate reaction can be stopped using 650 nm Liquid Stop Solution for TMB Microwell Substrates (LBSP). 3) Dilution of the substrate is not recommended. It is recommended that the antibodies or conjugates be diluted to reduce the intensity of the reaction. The product is not recommended for membrane or IHC applications that require a precipitating reaction product.

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