compound introduction
活性：>400单位/mg蛋白质。一个单位被定义为消化溶于10%TCA的血红蛋白释放肽的酶的量。通过在37℃下每60分钟增加1.0的A280来测量反应。 底物：酸变性血红蛋白（反应混合物中0.2%）。 缓冲液：0.1 M甲酸盐，pH 3.3。 Activity: >400 units/mg protein. One unit is defined as the amount of enzyme which digests hemoglobin releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37ºC.Substrate: acid-denatured hemoglobin (0.2% in reaction mixture).Buffer: 0.1 M formate, pH 3.3.
