UltraBio™ Anti-GFP Magnetic Beads (Anti-GFP磁珠)

阿拉丁生产的UltraBio™ Anti-GFP Magnetic Beads，即Anti-GFP磁珠，也称Anti-GFP免疫磁珠或GFP抗体磁珠，是由高品质的GFP小鼠单克隆抗体与纳米级氨基磁珠共价偶联而成，可特异性地与动植物或微生物裂解液、血清、腹水等中含有GFP标签的蛋白结合，从而用于带有GFP标签的融合蛋白或其蛋白复合物的免疫沉淀(Immunoprecipitation, IP)、免疫共沉淀(Co-IP)或纯化。GFP标签(GFP-tag)、Flag标签、Myc标签(Myc-tag)、HA标签(HA-tag)、 His标签(His-tag)和GST标签等是表达载体上最常见的一些标签，通过与这些标签的融合表达可以非常方便地检测目的蛋白及与目的蛋白相互结合的蛋白，也可以非常方便地用于目的蛋白的纯化。GFP (green fluorescent protein, 绿色荧光蛋白)最早是由下村修、钱永健等人于1962年在维多利亚多管发光水母(Aequorea victoria)中发现的，当受到紫外或蓝光激发时，发射绿色荧光。GFP或其突变体EGFP (enhanced green fluorescent protein, 增强型绿色荧光蛋白)与目的蛋白融合表达被广泛用于基团表达调控、转基因功能、目的蛋白在细胞中的表达、分布和迁移、以及高通量药物筛选等方面的研究。GFP标签的优点是便于观察，适合于观察蛋白在细胞内的定位，不用破碎组织细胞、不加任何底物、不用抗体，直接通过荧光显微镜就能在活细胞中观察到发出的绿色荧光，实时观察目的蛋白的表达情况，而且荧光性质稳定，细胞内的其它产物不会干扰GFP标签蛋白的检测，从而使其检测快速、简便、灵敏度高、重现性强，所以GFP被誉为活细胞探针。但GFP标签的分子量较大，约为25kDa，与目的基因形成融合蛋白时可能会影响目的蛋白的功能。UltraBio™ Anti-GFP Magnetic Beads (Anti-GFP磁珠)可以特异性地结合GFP或EGFP等标签融合蛋白，并可以借助磁力架等磁分离设备非常便捷地应用于带有GFP标签的融合蛋白或其蛋白复合物的免疫沉淀或纯化等实验。本产品进行免疫沉淀的流程参考图1。图1. 阿拉丁的UltraBio™ Anti-GFP Magnetic Beads (Anti-GFP磁珠)免疫沉淀流程图。本产品特异性强、靶蛋白结合量高。与国内外大多数的同类产品相比，本产品抗体结合密度高，对带有GFP标签蛋白的结合具有很强的特异性，并且本产品磁珠粒径小，不易产生非特异吸附。本产品每毫升磁珠悬浊液含约10mg磁珠，含有不少于0.6mg GFP抗体，通常可结合不少于0.6mg GFP标签融合蛋白，具体的最大结合量和标签蛋白的分子量大小等相关。每500微升样品，通常仅需使用10-20微升磁珠悬浊液，就可以高效地进行免疫沉淀实验。本产品可结合多种形式的GFP标签蛋白。本产品可特异性地结合N端GFP融合蛋白(GFP-Protein)、C端GFP融合蛋白(Protein-GFP)。本产品结合目的蛋白速度快。本产品使用了纳米级磁珠(~200nm)，具有超大的比表面积，便于抗体和抗原的快速有效结合。通常10分钟内即可完成抗原吸附的过程，30分钟内完成目的蛋白免疫沉淀操作。缩短操作时间可以有效避免在长时间操作过程中目的蛋白的降解或变性，充分保证目的蛋白的活性。本产品可选择多种洗脱方法。本产品可以根据目的蛋白的结构、生物学功能及后续应用的要求等，使用多种洗脱方法，包括SDS-PAGE上样缓冲液和酸性等洗脱液进行洗脱。本产品用于GFP-融合蛋白的免疫沉淀效果参考图2。图2. UltraBio™ Anti-GFP Magnetic Beads (Anti-GFP磁珠)用于GFP-融合蛋白的免疫沉淀效果图。293T (人胚肾细胞)转染含GFP-tag质粒36小时后，经Western及IP细胞裂解液裂解。样品1为Input，即全细胞裂解液(total)；样品2为Mouse IgG Magnetic Beads (小鼠IgG免疫磁珠) 免疫沉淀后经SDS-PAGE蛋白上样缓冲液(1X) 洗脱后的样品，该Mouse IgG是正常的小鼠IgG (Normal Mouse IgG)，为阴性对照；样品3为本磁珠免疫沉淀后的样品，该样品使用SDS-PAGE蛋白上样缓冲液(1X)洗脱。使用SDS-PAGE蛋白上样缓冲液(1X)洗脱后可以检测到GFP抗体的轻重链。Western印迹成像由UltraBio™ 600化学发光成像系统完成。实际结果会因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。本产品的主要指标如下表： 注意事项 ： 本Anti-GFP磁珠使用的GFP抗体的抗原为GFP蛋白的N端约20个氨基酸的多肽，由于GFP标签本身的分子量较大，约25kDa，与目的基因形成融合蛋白后分子量更大，而且融合蛋白的结构复杂性可能会影响Anti-GFP磁珠对GFP蛋白N端的识别，建议通过预实验进行免疫沉淀的验证或对裂解条件进行一定的调整。由于GFP-tag与GFP抗体的结合力非常强，酸性洗脱的效果可能比较差，建议优先使用SDS-PAGE上样缓冲液洗脱法。本产品需维持pH为6-8，避免高速离心和干燥；请勿长时间将磁珠置于磁场中，否则可能会引起磁珠聚团。本产品使用前要适当充分重悬，即颠倒若干次使磁珠混合均匀，混匀操作须轻柔，不宜剧烈涡旋震荡等，避免抗体变性等。在免疫沉淀或纯化时，建议设置阳性和阴性对照组。蛋白样品收集后宜尽快完成纯化工作，并应始终放置在4℃或冰浴，以减缓蛋白降解或变性。为有效抑制蛋白降解，可以在蛋白样品中添加适量的蛋白酶抑制剂混合物，例如阿拉丁的P1005/P1006蛋白酶抑制剂混合物(通用型)、P1048/P1049 蛋白酶磷酸酶抑制剂混合物(通用型, 质谱兼容, 50X)、P1010/P1011 蛋白酶抑制剂混合物(哺乳动物样品抽提用, 100X)、P1050/P1051 蛋白酶磷酸酶抑制剂混合物(哺乳动物样品抽提用, 50X)等。如果使用真空泵等仪器吸取上清液，须注意真空泵的吸液强度，以免吸力过大而吸取到聚集的磁珠。酸性溶液洗脱时磁珠可能会发生聚集，属于正常现象，不影响磁珠的正常使用。0.1%的非离子型去垢剂(如Triton X-100、Tween-20或NP-40)可有效防止磁珠聚集，并且不会影响磁珠的抗体结合效率。高浓度的DTT、巯基乙醇、盐酸胍等对本产品与标签蛋白的结合可能有一定影响，但Western及IP细胞裂解液、RIPA裂解液或NP-40裂解液等都完全适用。阿拉丁生产的不同裂解液的主要特点和差异，以及如何选择裂解液可参考我们的相关网页：http://www.aladdin-e.com/support/lysis-buffer.htm。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 1.样品的制备。a.选择合适的裂解液，用于制备细胞或组织的裂解液。优先推荐选择阿拉丁生产的P0013 Western及IP细胞裂解液用于细胞或组织样品的裂解。根据特定的实验目的，如有必要，也可以使用阿拉丁生产的P0013B RIPA裂解液(强)、P0013C RIPA裂解液(中)或P0013D RIPA裂解液(弱)用于样品的制备。如果使用自行配制的或其它公司生产的裂解液，需要确保裂解液的pH为6-8。注：本Anti-GFP磁珠使用的GFP抗体的抗原为GFP蛋白的N端约20个氨基酸的多肽，由于GFP标签本身的分子量较大，约25kDa，与目的基因形成融合蛋白后分子量更大，而且融合蛋白的结构复杂性可能会影响Anti-GFP磁珠对GFP蛋白N端的识别，建议通过预实验进行免疫沉淀的验证或对裂解条件进行一定的调整。b.具体的细胞或组织样品裂解的制备步骤请参考裂解液的使用说明。制备好的裂解液上清宜置于冰上或4℃存放，随后即可用于免疫沉淀或免疫共沉淀、标签蛋白的纯化等操作。新鲜制备好的样品，建议尽量当天完成免疫沉淀等后续操作，但如果样品不能当天使用，也可以适当分装后-80℃冻存。2.Anti-GFP磁珠的准备。由于Anti-GFP磁珠储存在特殊保护液中，所以需要在加入样品前适当洗涤。a.用移液器轻轻吹打重悬Anti-GFP磁珠，按照每500μl样品10μl或20μl磁珠悬浊液，取适量Anti-GFP磁珠至一洁净离心管中，加入1X TBS 至最终体积为约0.5ml。b.用移液器轻轻吹打并充分重悬Anti-GFP磁珠。置于磁力架(FMS012/FMS024)上分离10秒，去除上清。重复上述步骤两次。c.按照初始体积的量，用1X TBS 重悬Anti-GFP磁珠。3.免疫沉淀(Immunoprecipitation, IP):a.加入磁珠与孵育。按照每500μl蛋白样品加入10μl或20μl磁珠悬浊液的比例加入Anti-GFP磁珠，置于侧摆摇床或旋转混合仪上，室温孵育2小时或4℃孵育过夜。注：孵育过程中，如果磁珠发生聚团或呈片状属正常现象，不会影响实验结果。b.磁分离。孵育完毕后，置于磁力架上分离10秒，去除上清。注：可保留部分上清液，用于检测免疫沉淀的效果。c.洗涤。加入500μl的1X TBS，用移液器轻轻吹打重悬Anti-GFP磁珠。置于磁力架上分离10秒，去除上清。重复洗涤三次。注：也可以通过检测洗涤得到的洗涤液的OD280来判断是否洗涤完全，若OD280大于0.05，应适当增加洗涤次数。4.洗脱：根据标签蛋白的特点及后续实验要求，可以选择如下2种方法之一进行洗脱。a.SDS-PAGE上样缓冲液洗脱法：本方法为变性法，得到的蛋白样品适合SDS-PAGE电泳或WB检测。(a)SDS-PAGE上样缓冲液的配制：可以直接使用阿拉丁生产的 P0015A SDS-PAGE蛋白上样缓冲液(1X)，或使用阿拉丁生产的P0015 SDS-PAGE蛋白上样缓冲液(5X)或自行参考《分子克隆》等配制5X或2X的SDS-PAGE蛋白上样缓冲液，然后加入水配制成1X的SDS-PAGE蛋白上样缓冲液。通常SDS-PAGE蛋白上样缓冲液含有DTT等还原剂，其洗脱得到的蛋白样品中会含有GFP抗体的轻链和重链。(b)每10-20μl原始磁珠体积的磁珠，加入100μl 1X SDS-PAGE上样缓冲液，95℃加热5分钟。(c)置于磁力架上分离10秒，取上清进行SDS-PAGE电泳或Western检测。b.酸性洗脱法：本方法为非变性法，洗脱后的蛋白保持原有的生物活性，便于后续分析检测。(a)溶液的配制：酸性洗脱液(0.1M Glycine-HCl, pH3.0)，中和液(0.5M Tris-HCl, pH7.4, 1.5M NaCl)。(b)每10-20μl原始磁珠体积，加入100μl酸性洗脱液，混匀后置于侧摆摇床或旋转混合仪上，室温孵育5分钟。注：孵育时间不宜超过15分钟。注：洗脱液的体积可以酌情适当调整，同时须注意后续的中和液体积也需要作相应调整。(c)孵育完毕后，置于磁力架上分离10秒，将上清转移到新的离心管中，并加入10μl中和液，适当混匀。(d)为了获得最大的洗脱效率，可重复步骤b和c，并将相同样品合并。(e)洗脱并中和的GFP标签蛋白置于4℃待用，或者-20℃或-80℃长期保存。注1：由于GFP-tag与GFP抗体的结合力非常强，酸性洗脱的效果可能比较差，建议优先使用SDS-PAGE上样缓冲液洗脱法。注2：由于目的蛋白的差异可能对酸性洗脱法的洗脱效率有一定的影响，如果对洗脱效率的要求比较高，可对酸性洗脱液的pH在2.5-3.1之间进行一定的调整，相应的中和液的pH值或量也要进行一定的调整，例如100μl酸性洗脱液(0.1M Glycine-HCl, pH2.8)和15μl中和液(1M Tris-HCl, pH8.5)。常见问题： Problem Possible Causes Solution Very few or no GFP‐tagged protein exists in the eluate. Protein is not completely eluted. Change elution methods. No target protein expressed. Make sure the protein of interest contains the GFP-tag by Western blot or dot blot analyses. Very low protein expression level. 1. Use larger volume of cell lysate. 2. Optimize expression conditions to raise the protein expression level. Washes are too stringent. Reduce the time and number of washes. Incubation times are inadequate. Increase the incubation time. Interfering substance is present in sample. Lysates containing high concentration of DTT, 2-mercaptoethanol, or other reducing agents may destroy antibody function, and must be avoided. Detection system is inadequate. If Western blot detection is used: 1. Check primary and secondary antibodies using proper controls to confirm binding and reactivity.2. Verify that the transfer was adequate by using prestained protein marker or staining the membrane with Ponceau S.3. Use fresh detection substrate or try a different detection system. Background is too high. Proteins bind nonspecifically to the Anti-GFP monoclonal antibody, insufficient washing on magnetic beads, or the microcentrifuge tubes. 1. Pre-clear lysate with Mouse IgG Magnetic Beads to remove nonspecific binding proteins. 2. After suspending beads for the final wash, transfer entire sample to a clean microcentrifuge tube before centrifugation. Washes are insufficient. 1.Increase the number of washes.2.Prolong duration of the washes, incubating each wash for at least 15 minutes.3.Increase the salt and/or detergent concentrations in the wash solutions.4.Centrifuge at lower speed to avoid nonspecific trapping of denatured proteins. Multiple protein bands found in the eluate. The protein is not stable at room temperature. Purify the target protein at lower temperature, such as 4℃. Protein degradation due to proteases activity during purification process. Add protease inhibitors to cell lysate. Non‐specific binding. 1.Prepare cell lysate again.2.Add additional wash steps. Aladdin's UltraBio™ Anti-GFP Magnetic Beads are based on nano-scale magnetic beads covalently coupled with high-quality GFP mouse monoclonal antibodies that specifically bind to GFP-tagged proteins in animal, plant or microbial lysate, serum, ascites, etc. This product is mainly used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP) or purification of GFP-tagged fusion proteins or protein complexes.GFP (green fluorescent protein) was first discovered by Osamu Shimomura, Qian Yongjian and others in Aequorea victoria in 1962. When excited by ultraviolet or blue light, it emits green fluorescence. The fusion expression of GFP or its mutant EGFP (enhanced green fluorescent protein) and the target protein is widely used for gene expression regulation, transgene function, expression, distribution and migration of target protein in cells, high-throughput drug screening, etc. The advantage of the GFP label is that it is easy and suitable for real-time observation of the localization and expression of the target protein in the cell without the need of sample lysis and substrate or antibody additions. Moreover, the fluorescence of GFP is stable and other components in the cell generally do not interfere with the detection of GFP-tagged protein, so that the detection is fast, simple, sensitive, and reproducible. However, the molecular weight of the GFP tag is relatively large , which may affect the function of the target protein when fused with GFP.Store at 4℃ for up to 2 years, or at -20℃ for long term storage.UltraBio™ Anti-GFP Magnetic Beads specifically binds with proteins tagged with GFP or EGFP. With magnetic stand, this product can be conveniently used for immunoprecipitation or purification of fusion proteins or protein complexes with GFP tags. Please refer to Figure 1 for the IP assays with this product. Figure 1. The work flow of IP assays with the UltraBio™ Anti-GFP Magnetic Beads .This product has strong specificity and high binding capacity of target protein. Compared with most similar products, this product has higher binding capacity and specificity for GFP-tagged proteins, and the small particle size of magnetic beads makes it less prone to non-specific adsorption. Each milliliter of the beads suspension contains about 10mg magnetic beads coupled with no less than 0.6mg Anti-GFP antibody. It can bind no less than 0.6mg GFP-tagged proteins, which is usually dependent on the molecular weight of the tagged protein. Immunoprecipitation can be performed with 10-20μl of bead suspension for every 500μl of cell lysate.This product can bind to various forms of GFP-tagged protein. This product can specifically bind to both N-terminal GFP fusion protein (GFP-Protein) and C-terminal GFP fusion protein (Protein-GFP).This product binds to GFP-tagged proteins rapidlyt. This product uses nano-scale magnetic beads (~200nm) with a large specific surface area, enabling a fast and efficient binding between antibody and antigen. It usually takes 10 minutes for the binding and 30 minutes to complete the whole process of immunoprecitation, during which protein degradation and denaturation can be minimized effectively.This product is compatible with a variety of elution methods. The GFP-tagged protein can be eluted using SDS-PAGE loading buffer and acidic eluents, depending on the structure of the target protein, its biological function, and the requirements of subsequent applications. Please refer to Figure 2 for the IP result using this product. Figure 2. The immunoprecipitation effect of UltraBio™ Anti-GFP Magnetic Beads for GFP-tagged protein. 293T (human embryonic kidney) cells were transfected with the plasmid carrying the gene encoding GFP-tagged protein. After 36 hours post infection, cells were lysed using the Cell lysis buffer for Western and IP and immunoprecipation was performed with the cell lysate. Lane 1, Total cell lysate (Input); Lane 2, Mouse IgG Magnetic Beads negative control; Lane 3, IP results with the cell lysate and the UltraBio™ Anti-GFP Magnetic Beads , eluted with SDS-PAGE protein loading buffer (1X). The western membrane was blotted with anti-GFP antidody and examined by UltraBio™ 600 chemiluminescence imaging system . This figure is for reference only, which may vary due to different experimental conditions.This product is easy to use. This product is stored in a special protective solution free from glycerol, and can be quickly and efficiently separated from the solution by using a magnetic stand.Main parameters of the UltraBio™ Anti-GFP Magnetic Beads Precautions ： This product should be maintained at pH 6-8. Do not centrifuge, dry or freeze the magnetic beads. Long time exposure of the beads to magnetic field will cause beads to agglomerate.Resuspend Mag™ Anti-GFP Magnetic Beads evenly in solution by gentle pipetting prior to use. Do not vortex to avoid the denaturation of antibodies.When performing precipitation or purification, it is recommended to set up both positive and negative controls appropriately.The protein samples should be purified as soon as possible after collection and should always be placed at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by adding appropriate protease inhibitors, such as ’s Protease Inhibitor Cocktail for General Use , Protease and Phosphatase Inhibitor Cocktail for General Use (MS-safe, 50X) , Protease Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , Protease and Phosphatase Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , etc.If using a vacuum pump to aspirate the supernatant, the strength of the vacuum pump should be controlled properly to avoid the aspiration of magnetic beads.Magnetic beads may aggregate when eluted with acidic solutions, which is a normal phenomenon and does not affect the normal use of magnetic beads. 0.1% non-ionic detergent (such as Triton X-100, Tween-20 or NP-40) can effectively prevent magnetic beads from agglomerating without affecting the antibody binding efficiency of magnetic beads.High concentration of DTT, mercaptoethanol, guanidine hydrochloride, etc. may have a certain effect on the binding of this product to the tagged protein, but Cell lysis buffer for Western and IP , RIPA Lysis Buffer or NP-40 Lysis Buffer , etc. are completely applicable. The main features and differences of the different lysis solutions produced by , and how to choose the lysis solution can refer to our related webpage: http://www.aladdin-e.com/support/lysis-buffer.htm.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： 1. Sample preparationa. Lysis cells or tissues with appropriate lysis buffer. We recommend using the Cell Lysis Buffer for Western and IP in priority. Homemade or other company's lysis buffer with a pH of 6-8 can also be used.Note: The large molecular weight of GFP tag itself and the structural complexity of the fusion protein may affect the recognition of the N-terminal of the GFP protein by the Anti-GFP magnetic beads, so we recommend verifying the immunoprecipitation or adjusting the lysis conditions through preliminary experiments.b. Prepare the cell or tissue lysate according to the instructions of the lysis buffer. After lysis and centrifugation, keep the supernatant on ice or at 4ºC for subsequent use. We recommend performing subsequent procedures such as immunoprecipitation on the same day as the protein sample is prepared. Otherwise, make aliquot and store at -80ºC for future use.2. Preparation of Anti-GFP magnetic beadsSince Anti-GFP magnetic beads are stored in a special protective solution, they need to be washed properly before adding to samples.a. Resuspend the Magnetic Beads in the vial (gently pipette for 10 times, do not vortex). Transfer 10-20μl of Magnetic Beads suspension into a new tube (for 500μl of protein sample). The amount of beads suspension can be scaled up or down proportionally based on the volume of protein sample.Note: If using more than 0.2 ml of bead suspension, place the tube into a magnetic stand (FMS012/FMS024) to collect beads and remove the supernatant.b. Add 500μl of 1X TBS to the beads and pipette gently to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 2 times.c. Resuspend the Magnetic Beads with 1X TBS at an equal volume to the initial volume of beads suspension taken in step a (e.g. if 10μl beads suspension is taken, add 10μl of 1X TBS).3. Immunoprecipitation (IP)a. Incubation: Add 500μl of cell lysate to the washed beads, pipette gently to resuspend beads and incubate for 2 hours at room temperature or overnight at 4ºC while gently rotating the tube on a rotary mixer. Note: Magnetic beads may aggregate during incubation, which is a normal phenomenon and does not affect the result.b. Magnetic separation: Place the tube on a magnetic stand for 10 seconds to collect the beads against the side of the tube. Remove and discard the supernatant. Note: A small amount of supernatant can be reserved in a clean centrifuge tube for examination of the binding results.c. Wash: Add 500μl of 1X TBS to resuspend the beads by gently pipetting. Place the tube on a magnetic stand for 10 seconds to collect the beads and remove the supernatant. Repeat the wash 3 times. Note: The A280 of supernatant can be measured to determine whether the beads are washed thoroughly. Repeat wash until the A280 is smaller than 0.05.4. ElutionAccording to the characteristics of the tagged protein and subsequent experimental requirements, one of the following two methods can be selected for elution.a. Elution with 1X SDS-PAGE loading buffer under denaturing conditions. The eluate can be used for gel electrophoresis and immuoblotting. .(a)Preparation of SDS-PAGE loading buffer:The SDS-PAGE loading buffer can be homemade or purchased from Protein A Magnetic Beads1mlP2102-5ml Protein A Magnetic Beads5mlP2105-1ml Protein G Magnetic Beads1mlP2105-5ml Protein G Magnetic Beads5mlP2108-1ml Protein A+G Magnetic Beads1mlP2108-5ml Protein A+G Magnetic Beads5mlP2115-0.5ml Anti-Flag Magnetic Beads0.5mlP2115-2ml Anti-Flag Magnetic Beads2mlP2118-0.5ml Anti-Myc Magnetic Beads0.5mlP2118-10ml Anti-Myc Magnetic Beads10mlP2118-2ml Anti-Myc Magnetic Beads2mlP2121-0.5ml Anti-HA Magnetic Beads0.5mlP2135-0.5ml Anti-His Magnetic Beads0.5mlP2135-2ml Anti-His Magnetic Beads2mlP2138-0.5ml Anti-GST Magnetic Beads0.5mlP2138-2ml Anti-GST Magnetic Beads2mlP2141-0.5ml Anti-V5 Magnetic Beads0.5mlP2141-2ml Anti-V5 Magnetic Beads2mlP2151-1ml Streptavidin Magnetic Beads1mlP2151-200μl Streptavidin Magnetic Beads200μlP2151-5ml Streptavidin Magnetic Beads5mlP2171-1ml Mouse IgG Magnetic Beads1mlP2171-5ml Mouse IgG Magnetic Beads5mlP2173-1ml Rabbit IgG Magnetic Beads1mlP2173-5ml Rabbit IgG Magnetic Beads5ml

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