Singlet Oxygen Sensor Green Reagent

阿拉丁生产的SOSG(单线态氧绿色荧光探针)，即Singlet Oxygen Sensor Green Fluorescent Probe，也称Singlet Oxygen Sensor Green、Singlet Oxygen Sensor Green Reagent或单线态氧感应绿色荧光探针，是一种对单线态氧(Singlet oxygen, 1O2)具有高度选择性但不具细胞膜通透性的绿色荧光探针，常用于溶液体系中单线态氧的检测、自由基清除剂的功效评估等。SOSG 单线态氧荧光探针对于 1O2 具有高度选择性；与其它荧光或者化学发光单线态氧检测试剂不同，它对氢氧自由基(•OH)和超氧离子自由基(•O2-)无任何明显的响应。这种新型的单线态氧指示剂自身具有微弱的蓝色荧光，激发峰为 372 和 393nm，发射峰为 395 和 416nm。有单线态氧存在时，该探针可以发出与荧光素类似的绿色荧光(Ex/Em:504/525nm)。SOSG 单线态氧荧光探针试剂提供的是一种非细胞渗透性的衍生物。 SOSG 单线态氧荧光探针可用于水溶液中 1O2的检测，还可以用于评估自由基清除剂的功效，自由基清除剂常用于改善食品的风味和营养质量。需要注意的是 SOSG 单线态氧荧光探针在碱性 pH 条件下或者某些溶剂中会发生活化，溶剂包括但不限于乙腈、DMSO、DMF、丙酮等。随着时间的推移，其荧光产物在某些溶液中也会降解，但是条件控制得当的话，绿色荧光信号的强度可以与 1O2浓度相关，而不会受到其他活性氧的干扰。 注意事项： 1.荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。本产品建议在无菌环境中使用，否则可能会影响检测结果。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 2.收到产品后，立即将其干燥避光贮存于≤-20°C 冰箱；如需长期保存，建议贮存于-80℃冰箱。直到使用前取出。 使用说明： 1.SOSG甲醇储存液配制：取100µg本产品溶解于33µl甲醇中，即得约5mM的SOSG甲醇储存液。注：SOSG甲醇储存液分装后-80ºC储存，建议一个月内使用。 2.SOSG工作液配制：SOSG工作液应现用现配，可配制在适当的水溶液中，如超纯水或100mM Tris (pH7.5)等溶液中，最佳稀释缓冲液和工作浓度应根据实验需求确定，建议起始浓度范围为1-10µM。 3.在实验结束时请丢弃多余的SOSG工作液，不可保存后再用。 The SOSG (Singlet Oxygen Sensor Green Fluorescent Probe) produced by Aladdin, also known as Singlet Oxygen Sensor Green, Singlet Oxygen Sensor Green Reagent, or Singlet Oxygen Sensing Green Fluorescent Probe, is a highly selective green fluorescent probe for singlet oxygen (1O2) but lacks cell membrane permeability. It is commonly used for detecting singlet oxygen in solution systems and evaluating the efficacy of free radical scavengers. SOSG singlet oxygen fluorescent probe has high selectivity for 1O2; Unlike other fluorescent or chemiluminescence singlet oxygen detection reagents, it has no significant response to hydroxyl radicals (• OH) and superoxide ion radicals (• O2-). This new singlet oxygen indicator has weak blue fluorescence, with excitation peaks at 372 and 393nm and emission peaks at 395 and 416nm. When singlet oxygen is present, the probe can emit green fluorescence similar to fluorescein (Ex/Im: 504/525nm). The SOSG singlet oxygen fluorescent probe reagent provides a non cell permeable derivative. The SOSG singlet oxygen fluorescence probe can be used for the detection of 1O2 in aqueous solutions and can also be used to evaluate the efficacy of free radical scavengers, which are commonly used to improve the flavor and nutritional quality of food. It should be noted that the SOSG singlet oxygen fluorescent probe will undergo activation under alkaline pH conditions or in certain solvents, including but not limited to acetonitrile, DMSO, DMF, acetone, etc. Over time, its fluorescent products may also degrade in certain solutions, but if the conditions are properly controlled, the intensity of the green fluorescence signal can be correlated with the concentration of 1O2 without interference from other reactive oxygen species. Precautions ： 1.Fluorescent dyes are prone to quenching, so please avoid exposure to light to minimize fluorescence quenching.We recommend using this product in a sterile environment to ensure stable and consistent assay results.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. 2.After receiving the product, immediately dry it and store it in a refrigerator at ≤ -20°C away from light; If long-term storage is required, it is recommended to store in a -80℃ refrigerator. Remove it before use. Instructions for Use： 1.Preparation of SOSG Stock Solution in methanol: Dissolve 100µg of this product in 33µl of methanol to make a stock solution of approximately 5mM . Note: Aliquot and store the SOSG Stock Solution at -80ºC, and use within one month. 2.Preparation of SOSG Working Solution: The SOSG Working Solution should be prepared immediately before use in aqueous solution, such as ultrapure water or 100mM Tris (pH 7.5). The optimal working concentrations vary depending on experiments, with a suggested starting concentration range of 1-10µM. 3.Any excess SOSG working solution should be discarded, and can not be kept for later use.

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