ODN 1668

ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 is an immunostimulatory sequence and can be used as vaccine adjuvant. Sequence: 5'-tccatgacgttcctgatgct-3’ In Vitro ODN 1668 induces TNF-α secretion and promotes polyclonal B cell activation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. In Vivo ODN 1668 (10 nmol) stimulates responses to protein antigen . ODN 1668 (1 or 5 mg/kg; i.p. or s.c.; once) causes moderate fever and anorexia in rats. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: C57BW6 mice Dosage: 10 nmol Administration: 300 μg OVA (ovalbumin) in PBS or liposomes containing OVA were injected with or without 10nmol ODN in the hind footpads of C57BW6 mice. A boost of the same inoculum was given at 2 weeks, and 1 week later blood was taken for serum antibody titering. Result: Strongly potentiated the antibody response and induced class switching toward IgG2a and IgG2b. Showed B cell blast formation and a more than twofold increase in B7.2 (CD86) and IL-2 receptor-α (CD25) surface expression. Animal Model: Male Wistar rats with body weights in the range of 175–200 gDosage: 1 mg/kg or 5 mg/kg Administration: Intraperitoneal and subcutaneous injection, once Result: Caused moderate fever and anorexia. Induced a significant increase of IL-6. Increased expression of inflammatory genes and activated inflammatory transcription factors. Form:Solid IC50& Target:TLR9

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