E. Coli DNA Gyrase

Product Info Quality Control Tests: Nuclease contamination: assayed by testing for linear KDNA and linear plasmid DNA formation. Incubation of 1 µg of catenated KDNA or supercoiled pUC19 DNA for 4 hrs. at 37°C (under gyrase assay conditions and with or without ATP). Assay Conditions: Supercoiling assays are carried out using relaxed pBR-322 DNA under conditions specified in the protocol sheets provided with the product. We also perform assays using kDNA as substrate. For details on this decatenation/supercoiling assay please refer to our technical literature (link provided above). One unit of gyrase activity will supercoil 0.2 ug of plasmid in 30 min at 37°C. Included Materials: A 5X assay uffer and dilution buffer is included with the enzyme. These are also sold separately as 5X Gyrase Assay Buffer Gyrase Dilution Buffer (please enquire). Purification of Gyrase A and B Subunits Description Highly purified DNA Gyrase is offered as a holoenzyme (contains both A,B subunits). This is an excellent reagent for supercoiling plasmids in vitro or for novel drug screens. Shipping&storage The enzyme is shipped on dry ice and should be stored at -70°C. We also recommend that the enzyme be aliquoted after the first thaw (repeated rounds of freeze/thaw may cause loss of activity); the enzyme activity is stable for 1-3 days on ice.

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