脯氨酸二肽酶 来源于猪肾脏

来自猪肾脏的蛋白酶已用于：酶水解猪乳中的蛋白质和多肽中的 L-谷氨酰胺的回收脱脂乳蛋白水解法测定 ε-（γ-谷氨酰基）赖氨酸和游离氨基酸测定其对肠球菌素 A 2000 活性的影响脯氨酸蛋白酶在脯氨酸的循环利用和胶原蛋白的生产中具有重要作用。它被用来研究导致脯氨酸酶缺乏的 PEPD 基因的突变。它用于水解具有 C 末端脯氨酸或羟脯氨酸残基的蛋白质。在研究膜蛋白的酶促甲基化反应时，来自猪肾脏的脯氨酸蛋白酶（货号 P6675）已用于水解氨基末端的肽键。应用： 脯氨酸蛋白酶是一种胞内外肽酶。它是一种同二聚酶，在其活性位点中需要二价阳离子（如锰）作为辅因子才能发挥其功能。 Prolidase is a cytosolic exopeptidase. It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function. Application: Prolidase from porcine kidney has been used: in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids to determine its effect on the activity of enterococcin A 2000 Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins .

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