O-Glycoprotease是Pseudomonas aeruginosa 分泌的免疫金属蛋白酶(IMPa),特异性切割O-糖基化修饰的糖蛋白或糖肽中丝氨酸(S)或苏氨酸(T)肽键N-端。对于O-linked聚糖含唾液酸和不含唾液酸的粘蛋白S/T氨基酸没有选择性。用于O-糖基化修饰蛋白表征和糖蛋白O-糖苷结构的分析。 pH值范围: O-糖蛋白酶在pH7-8活性最佳 酶切方法: -适用酶切缓冲溶液:20mmol Tris or HEPES 缓冲液;pH值在7-8范围内 -去除表面活性剂的非变性或变性糖蛋白样品,按照20:1蛋白和蛋白酶的量使用,酶切反应蛋白浓度控制在0.2-0.5μg/μl,37℃干浴孵育2小时以上 -敏感性:O-糖蛋白酶对SDS,表面活性剂敏感;PH 缓冲液严格控制在7-8范围内,否则酶活性降低
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O-糖蛋白酶 (IMPa)(质谱级)
CAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 重组O 糖蛋白酶(质谱级) | O 糖蛋白酶(质谱级) | 免疫调节蛋白酶 | ||
| English alias | O glycoprotease | O-glycoprotease | IMPa | O glycoprotease | O-glycoprotease | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无载体 |
| PSA | - | logp | - |
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