Western Rapid Transfer Buffer (10X, Wet & Semi-dry)

阿拉丁的Western 快速转膜液(10×)是由阿拉丁生物自主研发的高效转膜液，可应用于湿转法转膜或者半干法转膜，能高效快速地将蛋白转移至 PVDF 膜或硝酸纤维素膜上，转移过程所产生的热量远远小于普通转膜缓冲液，从而减小对蛋白的损失。 本产品安全无毒害，使用前仅需用水和乙醇稀释至 1×(能在 15-35 分钟内快速完成转膜全过程)。 本产品转膜效率高，能在 15-35 分钟内快速完成转膜过程。本产品稳定性高，产热量小，减少对蛋白的损失。本产品兼容性好，对分子量跨度大的蛋白同样适用，有效解决大小蛋白不能在同一张膜上同时转移的问题，兼容 Tris-Gly 体系、Hepes 体系、Bis-Tris 体系等多种凝胶。 注意事项： 1. 当转膜液长时间在 2-8℃存放，若出现沉淀，可在水浴锅中温育并充分溶解后继续使用。 2. 注意在使用乙醇时，不要求必须使用无水乙醇，如使用含水乙醇，请按照试剂中乙醇实际浓度计算加入量。 3. 湿转转膜时电转仪外无需额外冰浴，即可确保无过热现象，如若希望更为快速地完成转膜，可使用 400mA 或更高的电流进行恒流转膜。 4. 本品仅供科研使用，勿做其他用途。 5. 为了您的安全和健康，请全程穿实验服并戴一次性手套操作。 使用说明： 1. 1×快速转膜液使用前的配制方法： 如配制 1L 的 1×快速转膜液请参考下表： 快速转膜液(10×) 100mL 无水乙醇 200mL 超纯水 700mL 准备 5-6 张裁剪好的滤纸和转印膜，如若使用 PVDF 膜需提前用无水甲醇浸泡至少10 秒以上，再放在 1×快速转膜液中。 2. 操作步骤及转膜条件： ①将滤纸和海绵浸泡于 1×快速转膜液中，务必完全浸湿。 ②将凝胶在超纯水中漂洗 2 分钟，以去除胶表面的 SDS；然后将凝胶浸泡于 1×快速转膜液中。 ③请注意：漂洗时间切勿超过 2 分钟，否则分子量较大的蛋白无法完全转移。 3. 请按照以下顺序完成转印三明治结构： →负极(阴极)→海绵→滤纸→凝胶→转印膜→滤纸→海绵(根据三明治的厚度选择是否使用) →正极(阳极) 将三明治结构转移至电泳槽中，设定恒流 400 mA，电泳时间 15-35 分钟完成蛋白转膜 Aladdin's Western rapid membrane transfer solution (10 ×) is an efficient membrane transfer solution independently developed by Aladdin Biotech, which can be used for wet or semi dry membrane transfer. It can efficiently and quickly transfer proteins to PVDF or nitrocellulose membranes, and the heat generated during the transfer process is much smaller than that of ordinary membrane transfer buffer, thereby reducing protein loss. This product is safe and non-toxic. Before use, it only needs to be diluted with water and ethanol to 1 x (which can quickly complete the entire film transfer process within 15-35 minutes). This product has high film transfer efficiency and can quickly complete the film transfer process within 15-35 minutes. This product has high stability, low heat generation, and reduces protein loss. This product has good compatibility and is also applicable to proteins with large molecular weight span. It can effectively solve the problem that large and small proteins cannot be transferred on the same membrane at the same time. It is compatible with Tris Gly system, Hepes system, Bis Tris system and other gel. Precautions： 1. When the transfer solution is stored at 2-8 ℃ for a long time, if precipitation occurs, it can be incubated in a water bath and fully dissolved before continuing to be used. 2. When using ethanol, it is not necessary to use anhydrous ethanol. If using aqueous ethanol, please calculate the amount added according to the actual concentration of ethanol in the reagent. 3. When wet rotating the film, there is no need for an additional ice bath outside the electrospinning device to ensure no overheating phenomenon. If you want to complete the film rotation more quickly, you can use a constant current of 400mA or higher to circulate the film. 4. This product is for scientific research purposes only and should not be used for any other purposes. 5. For your safety and health, please wear lab clothes and disposable gloves throughout the operation. Instructions for Use： 1. Preparation method for 1 x rapid transfer solution before use: Please refer to the table below for the preparation of 1L of 1 × rapid film transfer solution: Western Rapid Transfer Buffer (10X) 100mL Anhydrous ethanol 200mL Ultrapure water 700mL Prepare 5-6 cut filter papers and transfer films. If using PVDF film, soak it in anhydrous methanol for at least 10 seconds before placing it in 1 x rapid transfer solution. 2. Operation steps and film transfer conditions: ① Soak the filter paper and sponge in 1 x rapid film transfer solution, making sure they are completely soaked. ② Rinse the gel in ultrapure water for 2 minutes to remove SDS from the gel surface; Then immerse the gel in 1 × rapid membrane transfer solution. ③ Please note: The rinsing time should not exceed 2 minutes, otherwise proteins with higher molecular weight cannot be completely transferred. 3. Please complete the transfer sandwich structure in the following order: → Negative pole (cathode) → sponge → filter paper → gel → transfer film → filter paper → sponge (whether to use according to the thickness of sandwich) → positive pole (anode) Transfer the sandwich structure to the electrophoresis tank, set a constant current of 400 mA, and complete the protein transfer in 15-35 minutes for electrophoresis

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