Sincalide ammonium

Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium) is a rapid-acting amino acid polypeptide hormone analogue of cholecystokinin (CCK) for intravenous use in postevacuation cholecystography. Sincalide ammonium is a major bioactive segment of CCK that retains most of the biological activities of CCK. CCK‐8 can promote gallbladder contraction by injection and helps diagnose gallbladder and pancreas disorders. Sincalide ammonium can increase bile secretion, cause the gallbladder to contract and relax the sphincter of Oddi, resulting in bile drainage into the duodenum. Sincalide ammonium is a major bioactive segment of CCK that retains most of the biological activities of CCK . In Vitro Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium), as a novel cardiovascular hormone, has a significant inhibitory effect on myocardial fibrosis in noninfarcted areas. Sincalide ammonium also plays a positive role in fighting inflammation, apoptosis and collagen deposition. CCK‐8 (ammonium) protects H9c2 cardiomyoblasts from Ang II‐induced apoptosis partly via activation of the CCK1 receptor and the phosphatidyqinositol‐3 kinase/protein kinase B (PI3K/Akt) signaling pathway. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assaysup> Cell Line: H9c2 cells Concentration: 0.001, 0.01, 0.1, 1, 10, or 100 μmol/L Incubation Time: 24 h Result: Attenuated Ang II‐induced toxicity in H9c2 cells Apoptosis AnalysisCell Line: H9c2 cells Concentration: 0.001, 0.01, 0.1, 1, 10, or 100 μmol/L Incubation Time: 24 h Result: Decreased apoptotic cells, and prevented Ang II‐induced cytotoxicity that involves modulation of the PI3K/Akt pathway. Western Blot AnalysisCell Line: H9c2 cells Concentration: 0.001, 0.01, 0.1, 1, 10, or 100 μmol/L Incubation Time: 24 h Result: Expressed the protein and mRNAs of CCK and both its receptors in H9c2 cells. RT-PCRCell Line: H9c2 cells Concentration: 0.001, 0.01, 0.1, 1, 10, or 100 μmol/L Incubation Time: 24 h Result: Increased the protein and mRNA expression levels of CCK and decreased CCK 1 receptor expression levels at both the protein and mRNA levels with Ang II stimulation markedly. In Vivo Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium) alleviates fibrosis in the noninfarcted regions and delay the left ventricular remodeling and the progress of heart failure in a MI rat model. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: MI rat modelDosage: 50 μg/kg Administration: i.p.; 50 μg/kg/d; for 4 weeks Result: Had significant inhibitory effect on myocardial fibrosis in noninfarcted areas. Form:Solid

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