Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference. In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling diazo-labeled proteins : 1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature. 2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 °C for 18 h. 3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with λ ex =365 nm, λ em =480 nm. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid
All
Chemical Reagents
Biomedicine
Organic raw materials
Inorganic chemical industry
intermediate
Agricultural chemicals
Auxiliaries and catalysts
Fragrances and Flavors
Dyes and Pigments
Daily chemical industry
Fl-DIBO
CAS number：1407523-31-2 molecular formula：C19H12O3
overview
compound introduction
Specs
| Chinese alias | 氟迪博 | ||
| English alias | - | ||
| CAS number | 1407523-31-2 | molecular formula | C19H12O3 |
| molecular weight | 288.30 g/mol | Exact mass | ≥99% |
| PSA | - | logp | - |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
No safety information yet
Production methods and uses
No production method and use information yet
Related
upstream information
No upstream information yet
downstream information
No downstream information yet
MSDS
Found 0 shareMSDS
