compound introduction
MTSEA-荧光素是一种硫醇特异性荧光探针，可用于蛋白质拓扑结构和离子通道研究。MTS荧光团可用于实时监测构象变化。已经显示，只有当质子激活MTS化合物时，它才能不可逆地激活BNC1-G430C神经元阳离子通道。这表明荧光MTS试剂可用作细胞表面定位以及离子通道功能状态的报告基因。 MTSEA-Fluorescein is a thiol specific fluorescent probe useful for protein topology and ion channel research. MTS-fluorophores may be useful in the real-time monitoring of conformational changes. MTS compounds have been shown to irreversibly activate the BNC1-G430C neuronal cation channel only when the channel was activated by protons. This suggests that fluorescent MTS reagents may be used as reporters of cell surface localization as well as the functional status of ion channels.
