组织蛋白酶L,人肝

在20 mM丙二酸盐、pH 5.5、1 mM EDTA、400 mM NaCl中冷冻。 溶酶体蛋白酶中最强大的。在降解多种生理蛋白底物方面，它比组织蛋白酶B和H具有更高的比活性。组织蛋白酶L水平是原发性乳腺肿瘤治疗后复发和生存的有力预测因素。 活性：>1单位/mg蛋白质。一个单位定义为在Brij存在下，使用400 mM醋酸钠（pH 5.5）、4 mM EDTA和8 mM DTT作为活化缓冲液，在25℃下每分钟水解1微摩尔Z-Phe-Arg-AFC的酶量。 Frozen in 20 mM Malonate, pH 5.5, 1 mM EDTA, 400 mM NaCl. The most powerful of the lysosomal proteases. It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates. The level of cathepsin L is a strong predictor of relapse and survival following treatment of a primary breast tumor. Activity: > 1 unit/mg of protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Z-Phe-Arg-AFC per minute at 25oC using 400 mM Na Acetate, pH 5.5 with 4 mM EDTA and 8 mM DTT as the activation buffer in the presence of Brij. Product Citations: Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease." Neuromolecular medicine (2013): 1-11 Miller, Bailey, Aaron J. Friedman, Hyukjae Choi, James Hogan, J. Andrew McCammon, Vivian Hook, and William H. Gerwick. "The Marine Cyanobacterial Metabolite Gallinamide A Is a Potent and Selective Inhibitor of Human Cathepsin L." Journal of natural products (2013). References: Mason, R.W., Green, G.D.J. and Barrett, A.J. 1985. Biochem. J. 226, 233. Klijn et al. 1998. J. Clin. Onc.16, 1013. Barrett, A.J. and Kirschke, H. 1981. Methods Enzoymol. 80, 535. Tchope, J.R. et al. 1991. Biochem. Biophys. Acta. 1076. 149.

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