18S rRNA Cy3 FISH Probe

阿拉丁研发生产的18S rRNA Cy3 FISH Probe，即18S rRNA Cy3荧光原位杂交探针，是一种可以配合阿拉丁生产的Fluorescence in Situ Hybridization Kit for RNA 用于对来源于Human、Mouse、Rat、Prionailurus viverrinus、Tachysurus fulvidraco、Mugil cephalus和Neosciurus carolinensis (gray squirrel)的细胞或者固定的组织、石蜡或冰冻切片等样品的18S rRNA进行荧光原位杂交的通用型红色荧光探针。本产品可以用作荧光原位杂交(FISH)检测目的RNA时的阳性对照。阿拉丁的18S rRNA Cy3 FISH Probe是一种5’端进行了红色荧光染料Cy3标记的18S rRNA的21nt单链DNA序列，可以特异性识别18S rRNA 并进行杂交；其最大的激发波长为550nm，最大发射波长为570nm。本产品的推荐预杂交和杂交温度均为24℃。本产品杂交Hela细胞内18S rRNA效果请参考图1。图1. 阿拉丁研发生产的18S rRNA Cy3 FISH Probe 用于Hela 细胞18S rRNA的原位杂交的效果图。使用Fluorescence in Situ Hybridization Kit for RNA 进行原位杂交检测。图A为使用阴性对照探针0.5µg/ml Cy3-18S rRNA sense probe的FISH检测效果图，图B为DAPI染色效果图，图C为图A和图B的叠加图效果；图D为使用阳性红色荧光探针0.5µg/ml 18S rRNA Cy3 FISH Probe 即Cy3-18S rRNA antisense probe的FISH检测效果图，图E为DAPI染色效果图，图F为图D和图E的叠加效果图。Scale Bar均为20µm。实际结果会因实验条件、检测仪器等的不同而存在差异，图中效果仅供参考。18S rRNA Cy3 FISH Probe浓度为1mg/ml。用于荧光原位杂交时， 18S rRNA Cy3 FISH Probe的推荐工作浓度为0.5-1µg/ml。-20℃避光保存，一年有效。荧光探针均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 ： 本产品用于荧光原位杂交的具体操作请参见Fluorescence in Situ Hybridization Kit for RNA 的说明书。 Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus fulvidraco, Mugil cephalus, and Neosciurus carolinensis (gray squirrel) origin.This product can be used as a positive control in fluorescence in situ hybridization (FISH) assay of target RNA.This product is a 21nt single-stranded DNA sequence of 18S rRNA and can specifically recognize and hybridize 18S rRNA. It is labeled with the red fluorescent dye Cy3 at the 5' end, with a maximum excitation wavelength of 550nm and a maximum emission wavelength of 570nm.The recommended pre-hybridization and hybridization temperature for this product is 45ºC. Please refer to Figure 1 for the hybridization effect of this product on 18S rRNA in Hela cells.Figure 1. The in situ hybridization of 18S rRNA in Hela cells using Aladdin's 18S rRNA Cy3 FISH Probe and Fluorescence in Situ Hybridization Kit for RNA . A. Negative control assay with the Cy3-18S rRNA sense probe; B. Negative control cells stained with DAPI; C. The merge image of A and B; D. Assay of specimen with the 18S rRNA Cy3 FISH Probe ; E. Specimen stained with DAPI; F. The merge image of D and E. Scale Bars are 100µm. This figure is for reference only, which may vary due to different experimental conditions.The concentration of this product is 1mg/ml. For fluorescence in situ hybridization, the recommended working concentration of 18S rRNA Cy3 FISH Probe is 0.5-1µg/ml. Precautions ： This product should be protected from light to minimize the quenching of fluorescence.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use ： Please refer to the instructions of ’s Fluorescence in Situ Hybridization Kit for RNA .

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