Introduction: Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10⁻¹⁴ mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, microplate assays and microarrays. Conjugation: The R-PE was conjugated with Streptavidin under optimum conditions. Unconjugated streptavidin and mostly unconjugated R-PE were removed. Recommended Usage for Application： Flow: 0.02-1.25μg/mL Application Note: Centrifuge protein conjugate solutions briefly in a microcentrifuge before use and use only the supernatant for the experiment. This step will eliminate any protein aggregates that may have formed in solution, thereby reducing nonspecific background staining.
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Recombinant Streptavidin Protein (R-PE)
CAS number：unknown molecular formula：
overview
compound introduction
Specs
| Chinese alias | 链霉素亲和素 R-PE | 链霉亲和素 | 藻红蛋白结合物 | 链霉亲和素-R-藻红蛋白 | 链霉亲和素标记藻红蛋白(SA-PE) | R-藻红蛋白标记亲和素 | PE标记链霉亲和素 | 重组链霉亲和素蛋白 (R-PE) | ||
| English alias | SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | STREPTAVIDIN R-PHYCOERYTHRIN | Streptavidin-R-Phycoerythrin | PE Streptavidin | R-Phycoerythrin -conjugated Streptavidin | Streptavidin-Phycoerythrin (R-PE) | streptavidin | R-phycoerythrin | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | 无叠氮钠 |
| PSA | - | logp | - |
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