Protosappanin A (PTA), an immunosuppressive ingredient and major biphenyl compound isolated from Caesalpinia sappan L, suppresses JAK2/STAT3 -dependent inflammation pathway through down-regulating the phosphorylation of JAK2 and STAT3 In Vitro Protosappanin A (PTA: 12.5, 25, 50 μM, 24 hours) significantly inhibits the production of TNF-α and IL-1β in LPS-activated BV2 microglia. And the mRNA expressions of IL-6, IL-1β, and MCP-1 are reduced by PTA in a dose-dependent manner in BV2 microglial cell line. Protosappanin A (PTA: 12.5, 25, 50 μM, 24 hours) suppresses JAK2/STAT3-dependent inflammation pathway through down-regulating the phosphorylation of JAK2 and STAT3, as well as STAT3 nuclear translocation against LPS treatment. Protosappanin A (PTA: 12.5, 25, 50 μM, 24 hours) shows obvious effect on disturbing the interaction of transmembrane protein CD14 with Toll-like receptor-4, resulting in the inhibition of NF-κB-dependent oxidative and nitrative stress in LPS-induced BV2 microglia. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: Murine BV2 microglial cell line. Concentration: 12.5, 25, 50 μM. Incubation Time: 24 hours. Result: Inhibits the releases of NO, TNF-α and IL-1β in LPS-induced BV2 cells. Attenuated IL-6, IL-1β and MCP-1 gene expressions in the LPS-induced BV2 cells. Suppressed JAK2/STAT3 pathway activation in the LPS-induced BV2 cells. Form:Solid IC50& Target:JAK2 STAT3
All
Chemical Reagents
Biomedicine
Organic raw materials
Inorganic chemical industry
intermediate
Agricultural chemicals
Auxiliaries and catalysts
Fragrances and Flavors
Dyes and Pigments
Daily chemical industry
Protosappanin A
CAS number：102036-28-2 molecular formula：C15H12O5
overview
compound introduction
Specs
| Chinese alias | 原苏木素A | ||
| English alias | HY-113573 | CHEBI:69202 | MS-23852 | Q27137541 | 6H-Dibenz(b,d)oxocin-7(8H)-one, 3,10,11-trihydroxy- | 3,10,11-trihydroxy-7,8-dihydro-6h-dibenz[b,d]oxocin-7-one | AKOS040760044 | Protosappanin A | 5,14,15-trihydroxy-8-oxatricyclo[10.4.0.02,7]hexadeca-1(16 | ||
| CAS number | 102036-28-2 | molecular formula | C15H12O5 |
| molecular weight | 272.25 g/mol | Exact mass | ≥99% |
| PSA | 87.000 Ų | logp | 2.000 |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
No safety information yet
Production methods and uses
No production method and use information yet
Related
upstream information
No upstream information yet
downstream information
No downstream information yet
MSDS
Found 0 shareMSDS
