compound introduction
N-Acetylneuraminate lyase (NAL, EC 4.1.3.3) is a key member of the aldolase family that specifically catalyzes the reversible cleavage of N-acetylneuraminic acid (sialic acid) into N-acetylmannosamine and pyruvate. This enzyme directly participates in the synthetic and catabolic metabolic cycles of sialic acid within organisms, serving as a core enzyme in the sialic acid metabolic pathway. Reaction Properties Isoelectric point: 6.4 Michaelis constant: 2.5×10^-3 M (N-Acetylneuraminic acid) Optimum pH: 6.0~8.0 Fig. 1 Optimum temperature: 50℃ Fig. 3 pH Stability: 4.5~10 (25℃, 25hr) Fig. 2 Thermal stability:
