无蛋白快速封闭液

是否无菌：是 本产品是一种即用型，最新一代高效快速应用于Western Blot或ELISA等实验的封闭液，采用无蛋白配方，总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉、酪蛋白(Casein)等的封闭液及国外同类产品。该试剂不含有哺乳动物来源的蛋白成分且无内源的磷酸化蛋白及生物素，对磷酸化特异抗体及生物素也不会产生干扰。 注意事项： 1、不同抗体封闭时间有所不同，可根据具体实验进行操作，对于一些背景值相对较高的抗体，可适当延长封闭时间至30-60分钟，如有特殊需要，也可4°C过夜。 2、没有任何一种封闭液适用于所有实验体系，因此对于一些特殊的实验和抗体，可能需要根据具体情况考虑是否要更换为其他封闭剂，如BSA或者脱脂奶粉。 3、本产品仅供科研使用，勿做其他用途。 使用说明： 1、转膜完成后，用Western洗涤液洗涤蛋白膜约1-5分钟。 2、根据膜的大小，在平皿或其他适当的容器中加入封闭液，确保封闭液可以完全覆盖住膜，对于常规Western，一张7.5×8cm的膜推荐使用10ml左右的封闭液。 3、将膜用平角镊子轻轻夹到封闭液中，使封闭液完全覆盖住膜即可，置于摇床上摇晃封闭约10min。 4、取出封闭完成的膜，用洗涤液清洗蛋白膜2-3次，之后即可进行一抗稀释等Western Blot后续实验。 Is it sterile: Yes This product is a ready to use, latest generation efficient and rapid blocking solution for Western Blot or ELISA experiments. It uses a protein free formula and has a significantly better overall effect than traditional blocking solutions based on BSA (bovine serum albumin), skim milk powder, casein (Casein), and similar foreign products. This reagent does not contain protein components of mammalian origin and has no endogenous phosphorylated proteins or biotin. It also does not interfere with phosphorylated specific antibodies and biotin. Precautions： 1. The blocking time of different antibodies varies, and can be operated according to specific experiments. For antibodies with relatively high background values, the blocking time can be appropriately extended to 30-60 minutes. If there is a special need, it can also be left overnight at 4 ° C. 2. There is no single sealing solution suitable for all experimental systems, so for some special experiments and antibodies, it may be necessary to consider whether to replace them with other sealing agents, such as BSA or skim milk powder, depending on the specific situation. 3. This product is for scientific research purposes only and should not be used for any other purposes. Instructions for Use： 1. After the membrane transfer is completed, wash the protein membrane with Western wash solution for about 1-5 minutes. 2. According to the size of the membrane, add the sealing solution to a petri dish or other suitable container to ensure that the sealing solution can completely cover the membrane. For conventional Western blotting, it is recommended to use about 10ml of sealing solution for a 7.5 × 8cm membrane. 3. Gently clamp the membrane into the sealing solution using flat tweezers, ensuring that the sealing solution completely covers the membrane. Place it on a shaker and shake for about 10 minutes to seal. 4. Remove the sealed membrane and clean the protein membrane 2-3 times with washing solution, then proceed with Western Blot experiments such as dilution of primary antibody.

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