BM 化学发光 ELISA 底物 (POD)

BM化学发光ELISA底物(POD)为基于过氧化物酶（POD、HRP）的二级检测系统提供了底物溶液，它具有高度灵敏、化学发光增强的性能。该试剂已针对ELISA应用进行了优化，可在微孔板化学发光读取器（96孔格式）或管式发光仪上运行。应用： 酶联免疫吸附测定的灵敏度通常取决于所用比色底物的检测极限。 另外，由于吸光度测量的物理/化学限制，比色测定的动态范围被限制在最大两个数量级（朗伯比尔定律）。 利用化学发光技术，现在可以使用一种非放射性方法，该方法将基于试管或微孔板的免疫测定的便利性与同位素测定的优势相结合。 BM化学发光ELISA底物（POD）已在EG&G Berthold的LB 96 P酶标仪和LB 953管化学发光分析仪上进行了不同的免疫测定。 The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold. Application: The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.

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