STAMBP-IN-1

STAMBP-IN-1 is a small-molecule inhibitor of STAMBP deubiquitinase , and interrupts STAMBP-Ub-NALP7 interaction. STAMBP-IN-1 decreases protein level of its inflammasome substrate NALP7 and suppresses IL-1b release after Toll-like receptor (TLR) agonism. STAMBP-IN-1 inhibits the activity of STAMBP to cleave recombinant di-Ub with an IC 50 value of 0.33 mM. In Vitro STAMBP-IN-1 (0.1-10 μM; 6 h) exhibits the most potent ability to selectively decrease NALP7 abundance as well as endogenous NALP7 abundance in THP-1 cells, but not NALP6. STAMBP-IN-1 (0.01-100 μM; 37 ℃; 2 h) inhibits cleavage of K63-linked di-Ub (200 nM) to mono-Ub by purified recombinant STAMBP (25 nM) in a concentration dependent manner. STAMBP-IN-1 (0.01-10 μM; 37 ℃; 60 min) blocks STAMBP mediated deubiquitination of Ub-NALP7 in vitro in a concentration-dependent manner. STAMBP-IN-1 exhibits toxicity against THP-1 cells with an IC 50 of 106 μg/mL. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: THP-1 cells Concentration: 0.01, 0.1, 1, 10, and 100 μM Incubation Time: 6 hours Result: Inhibited the activity of STAMBP to cleave recombinant di-Ub in a concentrationdependent manner with an IC 50 of 0.33 mM (0.09-1.21 mM). Form:Solid

No numbering system information yet

No physical and chemical property information yet

No safety information yet

No production method and use information yet

No upstream information yet

No downstream information yet

No related product information yet