四甲基罗丹明异硫氰酸酯异构体R（TRITC）是四甲基罗丹明异硫氰酸酯的R异构体，也称6-四甲基罗丹明异硫氰酸酯。它是一种强荧光化合物，溶于乙醇、甲醇、二甲基甲酰胺、二甲亚砜和水。 TRITC是一种中等尺寸的氨基氧杂蒽染料，非平面的悬垂苯环上含有羧酸和异硫氰酸酯取代基。在酸性pH以及含亲水两性离子的近中性pH下，它形成亲脂阳离子。异硫氰酸酯基团与生物分子的亲核取代基（如氨基、羟基、巯基）反应，这为荧光标记的连接提供了方法。TRITC 也与水和氢氧离子反应，在水性溶液中不稳定。TRITC可以作为两性离子或氯化物在市面上买到。 应用 TRITC 用于标记各种生物分子，包括免疫球蛋白、 凝集素、核酸、多核苷酸和多糖，可用于亲和、 免疫和原位杂交、荧光探针和 流式细胞术。 凝集素是TRITC标记试剂，用于切片和细胞单层的亲和染色， 以识别唾液腺的唾液黏蛋白。 TRITC-抗体用于识别致病性变形虫。 TRITC-寡核苷酸用于土壤微生物的原位杂交染色 。 TRITC标记试剂也用作活细胞的荧光探针 。 TRITC标记粘菌细胞的流式细胞术已被用于研究 其聚集行为。 标记方法 （1）在0.1M，pH=9.0的Na2CO3溶液中配置蛋白的浓度至少2 mg/mL。 （2）在暗室中配置TRITC的干燥DMSO溶液，浓度为1 mg/mL；然后用铝箔包住避光。请勿使用旧的TRITC溶液，会影响标记效率。 （3）在暗室中慢慢加入50μL TRITC 到1mL 的蛋白溶液中，轻轻晃动混匀。 （4）在暗室中4 ° C 反应8小时以上；常温反应2～4小时。 （5）反应完毕加入100 mM NH4Cl 1 mL 再反应2小时淬灭未反应的TRITC。 （6）用PBS或其它缓冲液过柱纯化。 Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.
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6-TRITC, R-isomer [Tetramethylrhodamine-6-isothiocyanate]
CAS number：80724-20-5 molecular formula：C25H21N3O3S
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | Xanthylium, 9-(2-carboxyisothiocyanatophenyl)-3,6-bis(dimethylamino)- (inner salt) | Tetramethylrhodamine isothiocyanate | 2-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)-4-isothiocyanatobenzoate | 6-TRITC | 6-TRITC, R-isomer | tetramethylrhodami | ||
| CAS number | 80724-20-5 | molecular formula | C25H21N3O3S |
| molecular weight | 443.52 g/mol | Exact mass | ≥80% |
| PSA | 100.000 Ų | logp | 5.100 |
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MSDS
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80724-20-5 | Tetramethylrhodamine isothiocyanate IsomerR.pdf
