General description: Triton™ X-100是一种常用的非离子型的表面活性剂和乳化剂，经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂，并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。 Application: Triton™ X-100已用于： • 免疫组化用于平面视网膜的染色 • 与冰冷的PBS（磷酸盐缓冲液）一起悬浮细胞用于细胞DNA分析和Annexin V检测 • 在免疫荧光显微研究中对细胞进行通透化处理 • 在LDH检测中作为阳性对照用于测定细胞膜的完整性 • 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性 • 外层膜蛋白提取物的制备 • 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆 • 免疫荧光标记的组织切片处理 General description Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection Application Triton™ X-100 has been used: • In immunohistochemistry for staining the Flat-mount retinas • Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay • To permeabilise cells during Immunofluorescent microscopic studies • As a positive control in LDH assay to determine the cell membrane integrity • For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure • For the preparation of outer membrane protein exctract • As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells • In the treatment of tissue sections for Immunofluorescence labeling
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Triton™ X-100
Dangerous GoodsCAS number：9002-93-1 molecular formula：C8H17C6H4(OCH2CH2)nOH(n≈10)
overview
compound introduction
Specs
| Chinese alias | 4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇 | 聚乙二醇单-4-辛基苯基醚 | 聚乙二醇 叔辛基苯醚 | t-辛基苯氧基聚乙氧基乙醇 | ||
| English alias | Igepal CA-210 | PDSP1_001087 | Ethanol,1,3,3-tetramethylbutyl)phenoxy]- | Triton X 305 | P-TERT-OCTYLPHENYL (2-HYDROXYETHYL)ETHER | AT25387 | Igepal CA 630 | FT-0673247 | 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol | ||
| CAS number | 9002-93-1 | molecular formula | C8H17C6H4(OCH2CH2)nOH(n≈10) |
| molecular weight | 250.380 g/mol | Exact mass | 生化试剂 |
| PSA | 29.500 Ų | logp | 4.600 |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
pictogram：
GHS09 , GHS05 , GHS07
Signal word：
Danger
Hazard Statement：
H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H411: 对水生生物有毒并具有长期持续影响 H412: 对水生生物有害并具有长期持续影响 H302: 吞食有害 H318: 造成严重的眼睛损伤 H400: 对水生生物有剧毒 H410: 对水生生物有剧毒并具有长期持续影响
Statement of Preventive Measures：
P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。 P273: 避免释放到环境中。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P501: 将内容物/容器处理到。。。 P264: 处理后要彻底洗手。 P270: 使用本产品时，请勿进食、饮水或吸烟。 P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。 P391: 收集溢
WGK Germany：
1
Personal protective equipment：
Eyeshields,Faceshields,full-face respirator (US),Gloves,multi-purpose combination respirator cartridge (US),type ABEK (EN14387) respirator filter
RTECS：
MD0907700
UN Number：
3082
Production methods and uses
No production method and use information yet
Related
upstream information
No upstream information yet
downstream information
No downstream information yet
MSDS
Found 1 shareMSDS
9002-93-1 | Nonionic Surfactants | Triton ™ X-45
