5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH value (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nuclei and nuclear matrices.Fluorescence:λex 492 nm; λem 515 nm (Mercaptoethanol adduct);λex 493 nm; λem 522 nm (pH 9.2)Thiol-Reactive fluorescent probes such Iodoacetamides or maleimides are used for preparation of green-fluorescent thiol conjμgates of biomolecules. 5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH value (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nuclei and nuclear matrices.Fluorescence:λex 492 nm; λem 515 nm (Mercaptoethanol adduct);λex 493 nm; λem 522 nm (pH 9.2) Thiol-Reactive fluorescent probes such Iodoacetamides or maleimides are used for preparation of green-fluorescent thiol conjugates of biomolecules.
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5-Iodoacetamidofluorescein(5-IAF)
CAS number：63368-54-7 molecular formula：C22H14INO6
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| Chinese alias | 4(5)-碘乙酰氨基荧光素 | 5-(碘乙酰胺基)荧光素 | ||
| English alias | 5-IAF | HY-D0807 | LS-14922 | FT-0620512 | 5-Iodoacetamidofluorescein(5-IAF) | Flu-IA | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene], acetamide deriv. | SCHEMBL25103 | N-(3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-2-iodoacetamide | 5-i | ||
| CAS number | 63368-54-7 | molecular formula | C22H14INO6 |
| molecular weight | 515.254 g/mol | Exact mass | ≥95%(HPLC) |
| PSA | 105.000 Ų | logp | 3.300 |
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63368-54-7 | 5-(Iodoacetamido)fluorescein.pdf
