DAPI staining solution (ready to use)

产品介绍： DAPI是一种可对DNA染色的细胞核染色试剂，它在嵌入双链DNA后释放蓝色荧光，亮度增强约20倍。DAPI常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI具有很高的光漂白承受水平，能用来检测酵母线粒体DNA、叶绿体DNA、病毒DNA以及染色DNA等。 以贴壁细胞（96孔板）举例，每孔100 μL染色工作液，10 mL可以用于100个孔的染色。 产品参数： Ex/Em（结合DNA）=360/460 nm CAS号：28718-90-3 分子量：350.3 分子式：C16H17Cl2N5 注意事项： 1. 使用前请将产品瞬时离心至管底，再进行后续实验。 2. 本染色液的浓度经过优化的，可以满足各种常规染色的需要。如需调整使用浓度，请选择 D489987 或D106471，自行配置合适的工作液浓度。 3. DAPI对人体有害，请穿实验服并戴一次性手套操作，注意适当防护。 4. 荧光染料均存在淬灭问题，请尽量注意避光，以减缓淬灭。 应用范围： 细胞核染色 使用方法： 对于细胞或组织样品，固定后，适当洗涤去除固定剂。如果要进行免疫荧光染色，染色完成后再进行 DAPI 染色。如果不需要进行其他染色，则直接进行后续的 DAPI 染色。 1. 对于贴壁细胞（去除孔板中的培养基）或组织切片，加入少量 DAPI 染色液，覆盖住样品即可。对于悬浮细胞，至少加入待染色样品体积 3 倍的染色液，混匀。 2. 在室温培养细胞 10-20 min。 3. 用 PBS 或合适的缓冲液洗细胞两次，每次 3-5 min，洗涤完成后加入 50 μL PBS 防止细胞干掉。 4. 用带有 360 nm 激发波长，460 nm 发射波长的滤光片的荧光显微镜观察细胞。 Product introduction： DAPI is a nuclear staining reagent that can stain DNA. It releases blfluorescence after embedding double stranded DNA, and the brightness is enhanced about 20 times. DAPI is often used for apoptosis detection, and it is observed by fluorescence microscope or detected by flow cytometry after staining. DAPI has a high photobleaching tolerance and can be used to detect yeast mitochondrial DNA, chloroplast DNA, viral DNA and stained DNA. Take adherent cells (96 well plate) as an example, 100 per well μ L staining working solution, 10 ml can be used for the staining of 100 wells. Product parameters： Ex/em (bound DNA) =360/460 nmcas No.: 28718-90-3 molecular weight: 350.3 molecular formula: c16h17cl2n5 Matters needing attention： 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. the concentration of this dyeing solution is optimized to meet the needs of various conventional dyeing. If you need to adjust the concentration, please select D489987 or D106471 ，and configure the appropriate working fluid concentration by yourself. 3. DAPI is harmful to human body. Please wear experimental clothes and disposable gloves for operation, and pay attention to proper protection. 4. fluorescent dyes have quenching problems. Please try to avoid light to slow down quenching. Scope of application： Nuclear staining Instruction： For cell or tissue samples, after fixation, appropriate washing to remove the fixative. If immunofluorescence staining is performed, DAPI staining is performed after staining. If no other staining is required, subsequent DAPI staining is performed directly. 1.For adherent cells ( remove the medium in the orifice plate ) or tissue sections, add a small amount of DAPI staining solution and cover the sample. For suspension cells, at least 3 times the volume of the sample to be stained was added and mixed. 2.Cells were cultured at room temperature for 10-20 min. 3.The cells were washed twice with PBS or appropriate buffer, 3-5 min each time, and 50 μL PBS was added after washing to prevent the cells from drying out. 4.Cells were observed under a fluorescence microscope with a filter with 360 nm excitation wavelength and 460 nm emission wavelength.

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