用适当的过氧化物酶底物检测含有α-D-甘露糖、α-D-葡萄糖的糖蛋白通用Western Blot实验方法：糖蛋白样品规格：500ng凝集素浓度：0.1ug/ml每条泳道上样500 ng糖蛋白样品进行4-20% Bis-Tris SDS page凝胶转膜至PVDF膜上用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时用25ml RIPA缓冲液洗膜 5 x 5分钟用化学发光底物（CPS1-120）进行检测 Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate General Western Blot Protocol: Glycoprotein sample size: 500ng Lectin Concentration: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane Run 4-20% Bis-Tris SDS page gel Transfer gel to a PVDF membrane Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT Wash membrane 5 x 5 minutes with 25ml RIPA buffer Detect using chemiluminescent substrate (CPS1-120)
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伴刀豆球蛋白A 来源于洋刀豆 （刀豆）
CAS number：unknown molecular formula：
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | peroxidase conjugate |
| PSA | - | logp | - |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
pictogram：
GHS08 , GHS07
Signal word：
Danger
Hazard Statement：
H317: 可能引起皮肤过敏反应 H334: 吸入可能引起过敏或哮喘病症状或呼吸困难 H361: 怀疑破坏生育力或未出生的孩子
Statement of Preventive Measures：
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P302+P352: 如皮肤沾染：用水充分清洗。 P321: 特殊处理（请参阅此标签上的...）。 P405: 密闭存放 P501: 将内容物/容器处理到。。。 P284: 如果通风不良，请佩戴呼吸防护装置。 P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。 P272: 被污染的工作服不允许离开工作场所 P333+P313: 如发生皮肤刺激或皮疹：求医/就诊。 P362
WGK Germany：
3
Personal protective equipment：
dust mask type N95 (US),Eyeshields,Faceshields,Gloves
RTECS：
GK6890000
Production methods and uses
No production method and use information yet
Related
upstream information
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downstream information
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MSDS
Found 0 shareMSDS
