过氧化物酶 来源于辣根

辣根过氧化物酶已用于：粪便淀粉分析通过Trinder法测定血浆葡萄糖水平孵育阿魏酰基阿拉伯糖基木聚糖组分并评估氧化偶联检测糖蛋白应用： 辣根过氧化物酶是从辣根（Amoracia rusticana）中分离出来的，属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成，具体取决于特定同工酶。其分子量（约44 kDa）包括多肽链（33,890道尔顿）、氯化血红素加Ca2+ （约700道尔顿）和碳水化合物（约9,400道尔顿）。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。 Horseradish peroxidase is isolated from horseradish roots ( Amoracia rusticana ) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca 2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. Application: Peroxidase from horseradish has been used: in fecal starch analysis to measure plasma glucose levels by Trinder method to incubate feruloylated arabinoxylan fractions and also to evaluate oxidative coupling in the detection of glycoproteins

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