透明质酸酶（只降解透明质酸）

微生物来源的透明质酸（HA）降解酶属于多糖裂解酶，因此通常被称为HA裂解酶（HAase）（EC 4.2.2.1），该类酶通过β-消除反应断裂HA糖链中N-乙酰葡萄糖胺（GlcNAc）和葡萄糖醛酸（GlcUA）之间的β-1,4糖苷键，并在糖醛酸的C4和C5位上产生一个不饱和双键，该双键在232 nm有特征吸收，可以方便地用于寡糖产物分析检测。HAase已在多种细菌中被发现，如Proteus, Bacteroides fragilis, Streptococcus, Staphylococcus, Peptostreptococcus, Propionibacterium, Streptomyces, Clostridium, and Vibrio等。 许多HAase同时具有软骨素/硫酸软骨素降解活性，这类HAase常被归类到硫酸软骨素酶CSase AC。许多HAases在降解底物时起初表现为内切酶特点，对HA/CS多糖链进行随机结合并切割，随后则表现为外切酶特性，因此在底物降解过程中往往观测到不饱和二糖产物快速积累，但其它高聚合度寡糖占比则始终很低。 在我们的产品中，为了避免混淆，我们将同时具有HA和CS降解活性的HAases归入CSase AC，可以根据客户的需要提供各种具有不同比酶活和底物结构选择性的HAases。在此，HAase指的是来自于Streptomyces hyalurolyticus等的HA专一性降解酶，可以作为生物样品中HA分析检测和HA样品纯度检测的专一性工具酶。 Microbial derived hyaluronic acid (HA) degrading enzymes belong to polysaccharide lyases and are commonly referred to as HA lyases (EC 4.2.2.1). These enzymes break the β -1,4-glycosidic bond between N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcUA) in the HA sugar chain through a β - elimination reaction, and produce an unsaturated double bond at the C4 and C5 positions of the glucuronic acid. This double bond has characteristic absorption at 232 nm and can be conveniently used for oligosaccharide product analysis and detection. HAase has been found in various bacteria, such as Proteus, Bacteroides fragilis, Streptococcus, Staphylococcus, Peptostreptococcus, Propionibacterium, Streptomyces, Clostridium, and Vibrio Wait. Many HAases have both chondroitin sulfate/chondroitin sulfate degradation activity, and these HAases are often classified as chondroitin sulfate CSase AC. Many HAases initially exhibit endonuclease characteristics when degrading substrates, randomly binding and cleaving HA/CS polysaccharide chains, followed by exonuclease characteristics. Therefore, rapid accumulation of unsaturated disaccharide products is often observed during substrate degradation, while the proportion of other high degree of polymerization oligosaccharides remains low. In our product, to avoid confusion, we classify HAases with both HA and CS degradation activities as CSase AC. We can provide various HAases with different specific enzyme activities and substrate structure selectivity according to customer needs. Here, HAase refers to HA specific degrading enzymes from Streptomyces hyalurolyticus and others, which can be used as specific tool enzymes for HA analysis and purity detection in biological samples.

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