Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.Alkaline phosphatase is used for conjμgation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Optimum pH: 8.0 (Garen and Levinthal 1960). Extinction coefficient: E278 = 7.2 (Anderson et al. 1975). Isoelectric point: pH 4.5 (Garen and Levinthal 1960). Activity: Bloch and Schlesinger (1974) report on kinetic studies. Bock and Sheard (1975) indicate that the E. coli enzyme binds phosphate tightly over a wide range of pH forming complexes that may be intermediate in the hydrolytic action. See also Bosron and Vallee (1975), Zukin and Hollis (1975), and Chlebowski and Coleman (1974). Specificity: See review by Reid and Wilson (1971). Also Mushak and Coleman (1972) and Snyder and Wilson (1972). Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates. Stability: Stable at 2 - 8°C for at least 6 - 12 months. Chromatographically purified. A suspension in 2.6M ammonium sulfate, pH 8.0.
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磷酸酶(碱性)来源于大肠杆菌
Dangerous GoodsCAS number:unknown molecular formula:
overview
compound introduction
Specs
| Chinese alias | 碱性磷酸酯酶 | ||
| English alias | Alkaline phosphatase | 9001-78-9 | Phosphatase, alkaline | ENTEROSTATIN,HUMAN | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | - |
| PSA | - | logp | - |
numbering system
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physicochemical properties
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Safety information
pictogram:
GHS08 , GHS07
Signal word:
Warning
Hazard Statement:
H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H335: 可能引起呼吸道刺激 H317: 可能引起皮肤过敏反应 H334: 吸入可能引起过敏或哮喘病症状或呼吸困难
Statement of Preventive Measures:
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P305+P351+P338: 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。 P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。 P342+P311: 如有呼吸系统病症:呼叫急救中心/医生。
WGK Germany:
3
Personal protective equipment:
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
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MSDS
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