compound introduction
Creatinine Deiminase (CNI, EC 3.5.4.21) is a hydrolase that specifically catalyzes the ring-opening hydrolysis of creatinine, producing N-methylhydantoin and ammonia (NH₃). This enzyme is commonly found in microorganisms and is widely used in clinical diagnostics for the detection of creatinine levels. It serves as a key enzymatic component in vitro diagnostic reagents for renal function assessment. Reaction Properties Source: Microorganism Isoelectric point: 5.8 Michaelis constant: 6.6×10^-5 M (Creatinine) Optimum pH: 8.0 Fig. 1 Optimum temperature: 40~55℃ Fig. 3 pH Stability: 7.5~9.5 (30℃, 20hr) Fig. 2 Thermal stability:
