胶原酶 NB 7D 来源于溶组织梭菌

Collagenases from Clostridium histolyticum are proteolytic enzymes that cleave peptide bonds in the triple helical collagen molecule of human or animal tissue in situ. They are also referred as crude collagenases, because they consist of collagenase class I and class II, neutral proteases and clostripain in different ratios. The ratio of enzymatic activities can be altered enabling cell isolation from various tissue types. Collagenase NB 7D Gentle Grade is a partly purified collagenase that has slightly higher collagenase activity while containing lower tryptic activities. The product is intended for research use only. Specification Collagenase activity ≥ 0.2 U/mg (PZ acc. to Wünsch) Trypsin-like activities ≤ 0.2 U/mg (BAEE) Ratio Collagenase : Trypsin ≥ 3.0 Application Collagenase NB 7D Gentle Grade is suitable for dissociation of a broad variety of tissue types especially in cases where sensitive conditions are beneficial. Storage conditions Collagenase NB 7D Gentle Grade is provided as a lyophilized conditionspowder. It should be stored at +2 to +8 ℃ in a dry environment. Under these conditions the product is stable until the minimum shelf life stated on the certificate of analysis if repeated opening and closing of the vial is avoided. Instructions for use: General Collagenase NB 7D Gentle Grade is especially designed for isolation of sensitive cells (e.g. dendritic cells, diverse tumor cells) from human or animal tissues. The product exhibits slightly higher collagenase activity and low tryptic activities preserving cell-membrane receptors and other cell-surface proteins. Tissue dissociation Recommended starting concentrations for selected applications: Skin (mouse): 0.18 - 0.22 PZ U/ml Tumor cells (mouse): 0.50 - 0.60 PZ U/ml In general, the appropriate collagenase concentration depends on tissue type and origin as well as on the isolation procedure. Collagenase activity is at an optimum at 37 ℃ and pH 7.4. Working solution To prepare a working solution, the stock solution is diluted with buffer to achieve the required collagenase concentration. The working solu-tion must be constantly stored on ice until use. Inactivation and inhibitors The dissociation process can be reduced, e.g. by cooling down or dilution of the enzyme solution. Collagenase is reversibly inactivated at high pH values and irrevers-ibly inactivated at low pH values. Inhibitors of collagenase include cysteine or chelating agents like EDTA. Important note Collagenase NB 7D Gentle Grade is intended for research use only.

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