胰蛋白酶粉，猪源 1：250用于从组织培养板中释放粘附细胞进行传代。它被用于人内皮细胞的分离和培养。 对于胰蛋白酶消化肽，胰蛋白酶:肽的使用比例约为1:100至1:20。本品的典型用途是使贴壁细胞从培养表面脱落。 使细胞解离所需的胰蛋白酶浓度主要取决于细胞类型和培养物代数。 Trypsins也被用于细胞培养过程中的细胞再悬浮，在蛋白质组学研究中用于蛋白质的消化，以及在各种凝胶消化中。 其他的应用包括通过基于膜的技术评估结晶，以及在一项研究中确定蛋白质折叠速率和产量可以被动力学陷阱的存在所限制。 For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps. Trypsin Powder, Porcine 1:250 is used to release adherent cells from tissue culture plates for passaging. It was used in the isolation and culture of human endothelial cells.
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胰蛋白酶（猪源）
Dangerous GoodsCAS number：unknown molecular formula：
overview
compound introduction
Specs
| Chinese alias | - | ||
| English alias | - | ||
| CAS number | unknown | molecular formula | |
| molecular weight | - | Exact mass | - |
| PSA | - | logp | - |
numbering system
No numbering system information yet
physicochemical properties
No physical and chemical property information yet
Safety information
pictogram：
GHS08 , GHS07
Signal word：
Danger
Hazard Statement：
H315: 引起皮肤刺激 H319: 引起严重眼睛刺激 H335: 可能引起呼吸道刺激 H334: 吸入可能引起过敏或哮喘病症状或呼吸困难
Statement of Preventive Measures：
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾 P342+P311: 如有呼吸系统病症：呼叫急救中心/医生。
WGK Germany：
1
Personal protective equipment：
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RTECS：
YN5075000
Production methods and uses
No production method and use information yet
Related
upstream information
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downstream information
No downstream information yet
MSDS
Found 0 shareMSDS
