2,2′-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐（ABTS）已用作ELISA(酶联免疫吸附分析)的底物。ABTS还可用于测定包括血清在内的不同溶液中的葡萄糖浓度。食品工业使用ABTS来测定不同食品中的抗氧化能力。 由于它比TMB和OPD底物更不易被氧化，因此ABTS对ELISA应用的敏感性较低。因此，在背景结果较大的情况下，ABTS相较于TMB和OPD更具优势 推荐用于ELISA (微孔板)操作，不推荐用于膜应用。2,2′-联氮基-双3-乙基苯并噻唑啉-6-磺酸)是一种过氧化物酶底物，适用于ELISA程序。该底物产生一种可溶性最终产物，其颜色为绿色，可在405nm分光光度法下读取。用1%十二烷基硫酸钠(SDS)可使反应停止。 推荐用于ELISA (微孔)程序，不推荐用于膜应用。应用： ABTS主要可用于ELISA（酶联免疫吸附测定）实验中。ABTS，2，2′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）是一种生色底物，可用于：与漆酶或胆红素氧化酶的反应与过氧化物酶的反应：（例如HRP）。ABTS与过氧化氢结合 在过氧化氢的存在下，ABTS的过氧化物酶反应可产生绿色的可溶终产物，该终产物可以在405nm处用分光光度法进行读取。用1%十二烷基硫酸钠(SDS)可使反应停止。 ABTS，2，2′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐对光敏感，并且是一种水溶性化合物 ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures. ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used: For a reaction with laccase or bilirubin oxidase For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound Application: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).† ABTS is also used to measure glucose concentrations in different solutions including blood serum. The food industry uses ABTS to measure the antioxidant capacities within different foods. Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results It is recommended for ELISA (microwell) procedures, not recommended for membrane applications. 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.