N-Hydroxysuccinimide, commonly known as NHS, is a hydroxylated succinimide that is widely used in the activation of carboxylic acids to NHS-esters to promote coupling with nucleophiles. This approach is most commonly employed in activating the free acid forms of fluorophores for promoting conjμgation of these fluorophores to amine residues of proteins. Use of NHS ester activation has also been described in the synthesis of N-acyl amino acids.Useful in the carbodiimide method for improved amidations and peptide couplings. N-羟基琥珀酰亚胺可以在存在二环己基碳二亚胺的条件下在羧酸中通过脱水反应合成N-羟基琥珀酰亚胺酯。 N-羟基琥珀酰亚胺已用于中间介质的合成如： · N-丁二酰亚胺3-(二-三-丁基氟硅烷）苯甲酸 · 4-[2,2-双[(对-甲苯磺酰)-甲基]乙酰]苯甲酸-NHS酯 · N-丁二酰亚胺3-碘苯甲酸 它还用于微流体整合的表面等离子体共振（SPR）平台的微通道的表面修饰的实验方法中，用于对细菌病原体进行检测和定量。 碳二亚胺方法中使用的添加剂用于改良的酰胺化[1]及肽偶联。 N-Hydroxysuccinimide, commonly known as NHS, is a hydroxylated succinimide that is widely used in the activation of carboxylic acids to NHS-esters to promote coupling with nucleophiles. This approach is most commonly employed in activating the free acid forms of fluorophores for promoting conjugation of these fluorophores to amine residues of proteins. Use of NHS ester activation has also been described in the synthesis of N-acyl amino acids. Useful in the carbodiimide method for improved amidations and peptide couplings.