的3,3′,5,5′-四甲基联苯胺是超灵敏的ELISA液体底物，可用以下应用的作过氧化物酶底物：通过转染子测定合成的透明质酸和HAS2（透明质酸合成酶）表达测定结合的α-溶血素毒素的水平结合IgG成像3,3′,5,5′-四甲基联苯胺（TMB）是适用于ELISA程序的过氧化物酶底物。底物产生可溶的浅蓝色最终产物，并且可以在370或655nm下通过分光光度计读取。可以用2M H2SO4停止TMB反应（产生黄色溶液），并在450nm处读取数值。应用： 本品为包含3,3′,5,5′-四甲基联苯胺（TMB）的弱酸性缓冲液。底物以单组分即用型溶液提供。速率动力学约比传统TMB配方快40％。不推荐用于需要沉淀反应产物的膜或免疫组化应用。 This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product. Application: 3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase: to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant to measure the levels of α-hemolysin toxin in imaging bound IgGs 3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H 2 SO 4 (resulting in a yellow color), and read at 450 nm.