Ethoxyacetic acid is biomarker of exposure to widely used industrial chemicals such as alkyl-substituted ethylene glycols and other aliphatic ethers Ethoxyacetic acid is metabolite of glycol ether alkoxy acid and was used to evaluate its toxicity in embryonic stem cell test in mouse[3]. It was used in zebrafish embryotoxicity test to study chemical toxicity after exposure of the complete vertebrate embryo during embryogenesis in ovo[4]. application： Ethoxyacetic acid is used to evaluate toxicity in embryonic stem cell test in mice. It finds application as a biomarker of exposure to alkyl-substituted ethylene glycols and other aliphatic ethers. It is used as a precursor to prepare ethoxy-acetic acid ethoxymethyl ester.