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Product details
名称
Advanced DMEM/F-12
分子类型
培养基
别名
DMEM/F-12 (1:1) 减血清培养基 (Advanced) 含酚红、丙酮酸钠和非必需氨基酸,不含 L-谷氨酰胺和 HEPES
英文别名
DMEM/F-12 Reduced Serum Medium
英文名称
Advanced DMEM/F-12
货号
A1372075-500ml
包装规格
500ml
级别
无菌过滤
储存温度
2-8°C储存,避光
运输条件
冰袋运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
敏感性
Light-sensitive
产品介绍
Advanced DMEM/F-12(Dulbecco's Modified Eagle Medium/Ham's F-12)是一种广泛使用的减血清基础培养基,可在减少胎牛血清(FBS)添加量的情况下培养哺乳动物细胞。 Advanced DMEM/F-12培养基与其他基础培养基相比,之所以具有可减少血清添加量的效果,是因为添加了以下成分:胰岛素、转铁蛋白、牛血清白蛋白、乙醇胺、抗坏血酸、 谷胱甘肽以及多种微量元素(亚硒酸钠、偏钒酸铵、氯化锰和硫酸铜)。 与经典DMEM/F-12相比,在不影响细胞生长速率和形态的情况下,使用Advanced DMEM/F-12可减少50~90%的血清添加量,有助于降低培养条件波动,延长同一批次血清的使用时间。使用Advanced DMEM/F-12时,无需适应即可培养MRC-5、SP2、MSC、Vero、 CHO-K1、293T和Jurkat等细胞。 本产品含有:D-葡萄糖、非必需氨基酸、酚红、丙酮酸钠。 不含有:L-谷氨酰胺、HEPES。 使用说明: 1、使用前请添加1~5%的胎牛血清和4 mM L-谷氨酰胺或L-丙氨酰-L-谷氨酰胺。许多细胞系不需要适应即可使用本品。 2、需针对每种细胞系优化FBS浓度以较大限度减少血清添加量。请将本品培养的细胞放置于5% CO2的细胞培养箱。
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Research chemical 500ml

Research chemical 500ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 500ml.

item number:A1372075-500ml
Product model:500ml
level: 500ml
Lead Time:30days
sold 877 Items
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