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Product details

名称

Recombinant Taq DNA Polymerase Protein

别名

Taq DNA 聚合酶 | 不含DNA的Taq DNA 聚合酶 | 重组 Taq DNA 聚合酶蛋白

英文别名

DNA polymerase I | thermostable | DNA-directed DNA polymerase

货号

A156186-1000U

包装规格

1000U

级别

EnzymoPure™

浓度

≥95%

产品介绍

Taq DNA Polymerase简称Taq酶，是最常用的DNA聚合酶之一。 Taq DNA Polymerase是一种来源于嗜热菌Thermus aquaticus的高度热稳定的DNA聚合酶，分子量是94 kDa ，是将Thermus aquaticus DNA Polymerase 的基因重组表达后，分离提取而得到的。产品内容： 1. Taq DNA Polymerase ( 5 U/μL ) 2. 10×Taq Buffer（Mg2+Plus）：200mM Tris-HCl,500mM KCl,20mM MgCl2,1mM DTT,pH9.3 活性定义：一个单位的 Taq DNA 聚合酶是在74° C 下30分钟内将10nmol 脱氧核苷酸掺入 DNA 中所需的酶量。使用方法： 1. PCR反应体系试剂

生物活性

Taq DNA polymerase is a heat-resistant enzyme that synthesizes DNA from a single-stranded template in the presence of dNTP and primers, with 5´→3´ DNA polymerase activity and 5´→3´ exonuclease activity.

来源

重组表达

预测分子量

96 kDa

蛋白标签

N-His

SDS-PAGE

96 kDa

蛋白长度

Protein fragments

RIDADR

NONH for all modes of transport

表达系统

E. coli Accession #: P19821 | E. coli

氨基酸

1-832 aa

序列

MRGMLPLFEPKGRVLLVDGHHLAYRTFHALKGLTTSRGEPVQAVYGFAKSLLKALKEDGDAVIVVFDAKAPSFRHEAYGGYKAGRAPTPEDFPRQLALIKELVDLLGLARLEVPGYEADDVLASLAKKAEKEGYEVRILTADKDLYQLLSDRIHVLHPEGYLITPAWLWEKYGLRPDQWADYRALTGDESDNLPGVKGIGEKTARKLLEEWGSLEALLKNLDRLKPAIREKILAHMDDLKLSWDLAKVRTDLPLEVDFAKRREPDRERLRAFLERLEFGSLLHEFGLLESPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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