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Product details
名称
Anstart Taq II DNA Polymerase
别名
启动 Taq II DNA 聚合酶
货号
A292272-1KU
包装规格
1KU
级别
EnzymoPure™
浓度
5U/μL
产品介绍
热激条件:95℃,2.5min 外切酶活性:5’→3’ 防污染体系搭配:√ ARMS:√ SNP:√ 荧光定量PCR:√ 额外添加AT载体连接:√ 推荐项目:定性PCR、定量PCR 产品说明 Anstart Taq II DNA 聚合酶是抗 Taq单克隆抗体和 Taq II DNA 聚合酶的混合制品。是在原Anstart Taq DNA Polymerase (MD006)产品的升级款,具有更好的稳定性和特异性。高温加热前,抗Taq单克隆抗体与Taq聚合酶结合从而抑制聚合酶的活性,达到抑制低温条件下由引物的非特异性退火或引物二聚体引起的非特异性扩增作用。当扩增反应体系被加热到 95℃ 2min时,聚合酶活性因抗Taq单克隆抗体变性得到恢复,因此无需特殊失活处理,在常规PCR反应条件下即可使用。通过与改良的buffer配合使用,快速激活,能有效增加反应产物的量和提高PCR反应灵敏度和特异性。可应用与热启动PCR,扩增复杂模板,低拷贝靶片段扩增。 产品内容 1. Anstart Taq II DNA Polymerase ( 5 U/μl ) 2. 10×Anstart Taq II Buffer (without Mg2+ ) 3. 100mM MgCl2 产品用途 热启动法进行PCR 扩增。 使用方法 1.PCR 反应体系的设置 a.溶解并混匀 PCR 反应所需的各种溶液,并放置于冰浴上或冰盒内。建议反应 PCR 液体分装使用,避免反复冻融。 b.参考下表设置 PCR 反应,建议 PCR 反应体系的配置在冰浴或在冰盒上进行: 试剂
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Anstart Taq II DNA Polymerase 1KU

Anstart Taq II DNA Polymerase 1KU; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:A292272-1KU
Product model:1KU
level:
Lead Time:30days
sold 304 Items
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1KU

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