Ac-VLPE-FMK, a tetrapeptidyl mono-fluoromethyl ketone (m-FMK), is a Cat-B and Cat-L inhibitor. Ac-VLPE-FMK can be used for the research of cancer aggressiveness In Vitro Ac-VLPE-FMK (2 μM) significantly affects the cleavage of probe Ac-PLVQ-AMC by human recombinant CtsB and L. Ac-VLPE-FMK (30 min) inhibits the generation of the fluorescence derived from the cleavage of the substrate Ac-PLVQ-AMC in 769-p and A498 cells. Ac-VLPE-FMK (2.5-250 μM; 24-72 h) does not affect renal cancer cell viability, but influences cell migration rate, cellular adhesion, colony formation, and markers expression in renal cancer cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid IC50& Target:Cathepsin B cathepsin L