4-Aminoindole is an indole derivative. Its cytokinin activity has been assessed by tobacco pith callus bioassay.4-Aminoindole can be prepared by reacting 2,6-dinitrotoluene and N,N-dimethylformamide dimethylacetal in anhydrous DMF. Reactant for preparation of: ●Inhibitors of bacterial thymidylate synthase ●Mimetics of non-alkaloid toxin lignan anticancer and antiviral agent Podophyllotoxin (PPT) ●Inhibitors of Gli1-mediated transcription in the Hedgehog pathway ●Protein kinase C θ (PKCθ) inhibitors ●Indolic non-peptidic HIV protease inhibitors ●Transient receptor potential cation channel subfamily V member 1 (TRPV1) antagonists ●Cyclooxygenase-2 (COX-2) and lipoxygenase (LOX) inhibitors ●11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) inhibitors ●Short chain 4-substituted indoles as potent αvβ3 antagonist ●Ligands of serotonin transporter and 5-HT1A receptors